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MABS278 | Anti-EMAPII Antibody, clone M7/1, (Preservat ive free)

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ACT, IHC R Purified Monoclonal Antibody
      Catalogue NumberMABS278
      DescriptionAnti-EMAPII Antibody, clone M7/1, (Preservat ive free)
      Alternate Names
      • multisynthetase complex auxiliary component p43
      • multisynthetase complex auxiliary component 1
      • ARS-interacting multifunctional protein 1
      • Aminoacyl tRNA synthase complex-interacting multifunctional protein
      • Multisynthase complex auxiliary component p43
      • 1.Endothelial monocyte-activating polypeptide 2
      • EMAP-2
      • Endothelial monocyte-activating polypeptide II
      • EMAP-II
      • Small inducible cytokine subfamily E member 1
      Background InformationEndothelial monocyte-activating polypeptide II (EMAPII) is a 22 kDa pro-inflammatory cytokine that regulates TNFβ signaling. The mature EMAPII is formed from a functional EMAPII precursor protein in response to apoptosis, and other forms of cell stress. Previous studies have reported that it may decrease the growth of tumors by inhibiting angiogenesis. EMAPII has also been implicated in autoimmune diseases. However, EMAPII is also involved in a range of normal biological processes. It is a component of the multisynthase complex that binds tRNA and plays an essential role in protein translation, and may also play a role in lung development.
      Product Information
      • HeLa cell lysate
      PresentationPurified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.
      ApplicationAnti-EMAPII Antibody, clone M7/1, (Preservat ive free) detects level of EMAPII & has been published & validated for use in Western Blotting, Activity Assay & IHC.
      Key Applications
      • Western Blotting
      • Activity Assay
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in recombinant EMAP II proteins. (Rajashekhar, G., et al. (2009). J Immunol Methods. 350(1-2):22–28.)

      Neutralization Antibody: A representative lot was used by an independent laboratory to functionally neutralize pro and mature forms of EMAPII in lung tissue lysate. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in lung parenchyma of patients with emphysema. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to mouse EMAPII.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Aimp1
      • Emap2
      • Scye1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~43 kDa observed. This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected EMAPII in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EMAPII (Preservative free), -2544896 2544896
      Anti-EMAPII (Preservative free), -2622044 2622044
      Anti-EMAPII (Preservative free), -2665825 2665825
      Anti-EMAPII (Preservative free), -2687877 2687877
      Anti-EMAPII (Preservative free), -2720337 2720337
      Anti-EMAPII (Preservative free), -2816631 2816631
      Anti-EMAPII (Preservative free), clone M7/1 - Q2054377 Q2054377


      Reference overviewPub Med ID
      Lung endothelial monocyte-activating protein 2 is a mediator of cigarette smoke-induced emphysema in mice.
      Clauss, Matthias, et al.
      J. Clin. Invest., 121: 2470-9 (2011) 2011

      Show Abstract
      21576822 21576822
      A monoclonal rat anti-mouse EMAP II antibody that functionally neutralizes pro- and mature-EMAP II in vitro.
      Rajashekhar, Gangaraju, et al.
      J. Immunol. Methods, 350: 22-8 (2009) 2009

      Show Abstract
      19683532 19683532

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