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MABS835 | Anti-EGFR Antibody, clone 7F12.1

50 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, ICC, WB M Purified Monoclonal Antibody
      Catalogue NumberMABS835
      DescriptionAnti-EGFR Antibody, clone 7F12.1
      Alternate Names
      • Epidermal growth factor receptor
      • Proto-oncogene c-ErbB-1
      • Receptor tyrosine-protein kinase erbB-1
      Background InformationEpidermal growth factor receptor (EC; UniProt P00533; also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1, NISBD2) gene (Gene ID 1956) in human. The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands. Upon ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains, which allows its interaction with adaptor proteins and induction of several signaling pathways including PI3K-AKT; PLC-gamma-PKC; Notch1; and Ras-Raf. Hence, it contributes to a diverse range of cellular processes, including cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.
      Product Information
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect EGFR using this mouse monoclonal , Cat. No. MABS835, validated for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized A431 cells.

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected plasma membrane EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.
      Biological Information
      ImmunogenGST-tagged recombinant human EGFR fragment correspoding to a cytoplasmic region C-terminal to the kinase domain.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityEpitope region is present in spliced isoform 1 (p170), but not in isoform 2 (p60, Truncated, TEGFR), isoform 3 (p110) or isoform 4 reported by UniProt (P00533).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB
      • ERBB1
      • HER1
      • NISBD2
      UniProt Number
      Molecular Weight~170 kDa observed. Target band size appears larger than the calculated molecualar weight of 134.3 kDa due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected EGFR in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 μg
      Transport Information
      Supplemental Information