ST1513 Anti-EDN3 Mouse mAb (2A6-2A4)

ST1513
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1513-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~26 kDa EDN3 protein in LNCaP cells.
          Catalogue NumberST1513
          Brand Family Calbiochem®
          SynonymsAnti-Endothelin 3
          Application Data
          Detection of human EDN3 by immunoblotting. Sample: Whole cell lysate from LNCaP cells. Primary antibody: Anti-EDN3 Mouse mAb (2A6-2A4) (Cat. No. ST1513) (1-5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlLNCaP cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
          Clone2A6-2A4
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-EDN3 Mouse mAb (2A6-2A4) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-November-2010 RFH
          SynonymsAnti-Endothelin 3
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application Data
          Detection of human EDN3 by immunoblotting. Sample: Whole cell lysate from LNCaP cells. Primary antibody: Anti-EDN3 Mouse mAb (2A6-2A4) (Cat. No. ST1513) (1-5 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~26 kDa EDN3 protein.
          BackgroundThe protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons.
          HostMouse
          ImmunogenFull-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
          Clone2A6-2A4
          IsotypeIgG₁
          Specieshuman
          Positive controlLNCaP cells
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review