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MABT371 | Anti-EB1, EB2 and EB3 Antibody, clone KT65

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC, IP R Purified Monoclonal Antibody
      Catalogue NumberMABT371
      DescriptionAnti-EB1, EB2 and EB3 Antibody, clone KT65
      Alternate Names
      • Microtubule-associated protein RP/EB family member 1
      • APC-binding protein EB1
      • End-binding protein 1
      • EB1
      • EB2
      • End binding protein 2
      • EB3
      • End binding protein 3
      • Microtubule-associated protein RP/EB family member 3
      Background InformationEB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPRE3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play major roles in cellular migration and adhesion, and are thought to associate with adenomatous polyposis coli tumor suppressor.
      Product Information
      PresentationPurified rat monoclonal IgG2aκ in buffer containing PBS.
      ApplicationThis Anti-EB1, EB2 & EB3 Antibody, clone KT65 is validated for use in western blotting, ICC & IP for the detection of EB1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A representative lot detected EB1, EB2, and EB3 in NIH/3T3 cells (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated EB1, EB2, and EB3 in HeLa cell extracts (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse EB1, EB2, and EB3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody is expected to detect EB1, EB2 and EB3.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mapre1
      • EB2
      • MAPRE2
      • EB3
      • RP3
      • EBF3-S2
      • EBF3
      • MAPRE3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight31, 37, 32 kDa calculated. The calculated MW of EB1 (31 kDa), EB2 (37 kDa), and EB3 (32 kDa). The recombinants used to test the reactivity of this antibody to EB1, EB2, and EB3 have the following MW's. EB1 (56 kDa), EB2 (62 kDa), and EB3 (58 kDa).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected EB1, EB2, and EB3 in 10 µg of MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EB1, EB2 and EB3, clone KT65 - QVP1310183 QVP1310183


      Reference overviewPub Med ID
      SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase.
      van der Vaart, Babet, et al.
      J. Cell Biol., 193: 1083-99 (2011) 2011

      Show Abstract
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