A part of MilliporeSigma

MABE949 | Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB M Purified Monoclonal Antibody
      Catalogue NumberMABE949
      DescriptionAnti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1
      Alternate Names
      • E3 ubiquitin-protein ligase BRE1B
      • 95 kDa retinoblastoma-associated protein
      • BRE1-B
      • RBP95
      • RING finger protein 40
      • Syntaxin-1-interacting RING finger protein
      • Protein staring
      Background InformationE3 ubiquitin-protein ligase BRE1B (UniProt O75150; also known as 95 kDa retinoblastoma-associated protein, BRE1-B, RBP95, RING finger protein 40, Syntaxin-1-interacting RING finger protein, Protein staring) is encoded by the RNF40 (also known as BRE1B, KIAA0661) gene (Gene ID 9810) in human. Proteini ubiquitination is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. RING finger ubiquitin ligase members can function as monomers, dimers, or multi-subunit complexes. The RNF20-RNF40 heterodimer mediates the ubiquitination of histone H2B at Lysine 120, an event that plays an important role in decompacting chromatin fiber to allow transcription elongation, in stem cell differentiation, as well as in DNA double-strand breaks (DSBs) repair via the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. Human RNF40 is a 1001-amino acid protein containing a RING-type zinc finger in its C-terminal region (a.a. 948-987) that mediates its interaction with RNF20.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1 is an antibody against E3 Ubiquitin Ligase BRE1B for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment from the C-terminal half of human BRE1B .
      EpitopeInternal (C-terminal half).
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 4H8.1 epitope region is present in all three spliced isoforms of human BRE1B reported by UniProt (O75150).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF40
      • BRE1B
      • KIAA0661
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~120 kDa oberved. 113.7 kDa (isoform 1), 94.80 kDa (isoform 2), 102.0 kDa (isoform 3) calculated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BRE1B in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information