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MABT218 | Anti-E-Cadherin, clone 9E3.1 Antibody

MABT218
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT218
      Replaces04-1103
      DescriptionAnti-E-Cadherin, clone 9E3.1 Antibody
      Alternate Names
      • Cadherin-1
      • CAM 120/80
      • Epithelial cadherin
      • E-cadherin
      • Uvomorulin
      Background InformationCadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-E-Cadherin antibody, clone 9E3.1 is an antibody against E-Cadherin for use in western blotting & flow cytometry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 2 µg of this antibody detected E-Cadherin-1 in 1X10E6 MCF-7 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the extracellular domain of human E-Cadherin-1.
      EpitopeThis antibody recognizes the extracellular domain of E-Cadherin.
      Clone9E3.1
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH1
      • CDHE
      • UVO
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110 kDa observed. The calculated molecular weight is 97 kDa; however, this protein has been observed at ~110 kDa. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected E-Cadherin-1 in 200 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies