MAB1618 Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

MAB1618
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Dr, Sh, F, Xn ICC, IF, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1618
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1
      Alternate Names
      • Cytoplasmic dynein 1 intermediate chain 1
      • Cytoplasmic dynein intermediate chain 1
      • Dynein intermediate chain 1, cytosolic
      • DH IC-1
      • Cytoplasmic dynein 1 intermediate chain 2
      • Cytoplasmic dynein intermediate chain 2
      • Dynein intermediate chain 2, cytosolic
      • DH IC-2
      Background InformationCytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cells, A431 cell lysate
      PresentationPurified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      Applications
      ApplicationAnti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 detects level of Dynein & has been published & validated for use in IC, IF, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry:
      1:50-1:100 dilution from a previous lot was used. Reacts with cultured MDCK cells, NRR cells, N1E cells, and PTK-1 cells.

      Immunoprecipitation:
      10 μL of a previous lot per 0.5 gm tissue was used. The monoclonal cleanly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

      Immunoblotting:
      1:1,000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine brain cytoplasmic dynein.
      EpitopeWithin N-terminal 60 amino acids.
      Clone74.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with all known forms of the Mr = 74,000 Dalton intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes).
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Drosophila
      • Sheep
      • Fish
      • Xenopus
      Species Reactivity NoteRat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid. Not tested on chicken, nematodes or Dictystelium.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DYNC1I1
      • DYNC1I2
      Non-Reactive Species
      • Squid
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~74 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected DYNEIN on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Dynein, 74 kDa Intermediate -25576582557658
      Anti-Dynein, 74 kDa Intermediate -27819002781900
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 24305032430503
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 19732211973221
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 20111342011134
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 20439002043900
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 21933112193311
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 22938382293838
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 24890222489022
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - 31984533198453

      References

      Reference overviewApplicationPub Med ID
      Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane.
      Brandstaetter, H; Kruppa, AJ; Buss, F
      Disease models & mechanisms  7  1335-40  2014

      Show Abstract
      25368120 25368120
      Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis.
      Song, H; Bush, RA; Vijayasarathy, C; Fariss, RN; Kjellstrom, S; Sieving, PA
      Investigative ophthalmology & visual science  55  2659-68  2014

      Show Abstract
      Immunohistochemistry24651551 24651551
      Motor-driven marginal band coiling promotes cell shape change during platelet activation.
      Diagouraga, B; Grichine, A; Fertin, A; Wang, J; Khochbin, S; Sadoul, K
      The Journal of cell biology  204  177-85  2014

      Show Abstract
      24421335 24421335
      Subunit composition of the human cytoplasmic dynein-2 complex.
      Asante, D; Stevenson, NL; Stephens, DJ
      Journal of cell science  127  4774-87  2014

      Show Abstract
      25205765 25205765
      Nigrostriatal dynein changes in A53T alpha-synuclein transgenic mice.
      Liu, Y; Yuan, YH; Sun, JD; Li, J; Li, ZP; Chen, NH
      F1000Research  3  68  2014

      Show Abstract
      25210616 25210616
      The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation.
      Schwarz, DS; Blower, MD
      The Journal of cell biology  207  41-57  2014

      Show Abstract
      25287301 25287301
      Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions.
      Zhang, J; Twelvetrees, AE; Lazarus, JE; Blasier, KR; Yao, X; Inamdar, NA; Holzbaur, EL; Pfister, KK; Xiang, X
      Cytoskeleton (Hoboken, N.J.)  70  215-27  2013

      Show Abstract
      23475693 23475693
      Requirement for protein kinase A in the phosphorylation of the TGFβ receptor-interacting protein km23-1 as a component of TGFβ downstream effects.
      Jin, Q; Zhong, Y; Mulder, KM
      Experimental cell research  319  897-907  2013

      Show Abstract
      23333499 23333499
      Dynein interacts with the neural cell adhesion molecule (NCAM180) to tether dynamic microtubules and maintain synaptic density in cortical neurons.
      Perlson, E; Hendricks, AG; Lazarus, JE; Ben-Yaakov, K; Gradus, T; Tokito, M; Holzbaur, EL
      The Journal of biological chemistry  288  27812-24  2013

      Show Abstract
      23960070 23960070
      Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.
      Kong, S; Du, X; Du, X; Peng, C; Wu, Y; Li, H; Jin, X; Hou, L; Deng, K; Xu, T; Tao, W
      Cell research  23  835-50  2013

      Show Abstract
      23628724 23628724

      Data Sheet

      Title
      Anti-Dynein, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies