AB2231 | Anti-Dopamine Transporter; 100 µg Antibody

AB2231
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB2231
      DescriptionAnti-Dopamine Transporter; 100 µg Antibody
      Alternate Names
      • DA transporter
      • dopamine transporter
      • solute carrier family 6 (neurotransmitter transporter, dopamine),member 3, variable number tandem repeat (VNTR)
      Background InformationPlasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT), a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction. DAT is also the target of several “DAT-blockers” including amphetamines and cocaine. These chemicals inhibit the action of DAT and, to a lesser extent, the other monoamine transporters, but their effects are mediated by separate mechanisms.
      References
      Product Information
      FormatPurified
      Control
      • Rat Brain Lysate.
      PresentationPurified Antibody in PBS with 0.1% NaN3
      Applications
      ApplicationThis Anti-Dopamine Transporter, 100 µg Antibody is validated for use in WB for the detection of Dopamine Transporter, 100 µg.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic Linear Peptide
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityCat. AB2231 recognizes the N-terminus of the reduced and non reduced forms of DAT.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM]
      Gene Symbol
      • DAT
      • DAT 1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
      SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
      SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) family.
      Molecular Weight~ 50 – 80 kDa
      Reduced form at approx 50 kDa and Non-reduced form at approx. 80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinelyevaluated by western blot on rat brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Dopamine Transporter - 2430425 2430425
      Anti-Dopamine Transporter - 1944113 1944113
      Anti-Dopamine Transporter - 2016761 2016761
      Anti-Dopamine Transporter - 2109401 2109401
      Anti-Dopamine Transporter - 2202447 2202447
      Anti-Dopamine Transporter - 2318782 2318782
      Anti-Dopamine Transporter - JBC1771121 JBC1771121
      Anti-Dopamine Transporter - NG1853750 NG1853750
      Anti-Dopamine Transporter - NG1900345 NG1900345
      Anti-Dopamine Transporter -2586474 2586474

      References

      Reference overviewApplicationSpeciesPub Med ID
      Prepuberal stimulation of 5-HT7-R by LP-211 in a rat model of hyper-activity and attention-deficit: permanent effects on attention, brain amino acids and synaptic markers in the fronto-striatal interface.
      Ruocco, LA; Treno, C; Gironi Carnevale, UA; Arra, C; Boatto, G; Nieddu, M; Pagano, C; Illiano, P; Barbato, F; Tino, A; Carboni, E; Laviola, G; Lacivita, E; Leopoldo, M; Adriani, W; Sadile, AG
      PloS one  9  e83003  2014

      Show Abstract
      Western Blotting24709857 24709857
      Dopaminergic dysregulation in prefrontal cortex of rhesus monkeys following cocaine self-administration.
      McIntosh, S; Howell, L; Hemby, SE
      Frontiers in psychiatry  4  88  2013

      Show Abstract
      23970867 23970867
      Prolonged high fat diet reduces dopamine reuptake without altering DAT gene expression.
      Cone, JJ; Chartoff, EH; Potter, DN; Ebner, SR; Roitman, MF
      PloS one  8  e58251  2013

      Show Abstract
      Western Blotting23516454 23516454
      Traumatic brain injury and trichloroethylene exposure interact and produce functional, histological, and mitochondrial deficits.
      Sauerbeck, A; Hunter, R; Bing, G; Sullivan, PG
      Experimental neurology  234  85-94  2012

      Show Abstract
      22201550 22201550
      alpha -Synucleinopathy and selective dopaminergic neuron loss in a rat lentiviral-based model of Parkinson's disease.
      Lo Bianco, C, et al.
      Proc. Natl. Acad. Sci. U.S.A., 99: 10813-8 (2002)  2002

      12122208 12122208
      Protein kinase C-mediated functional regulation of dopamine transporter is not achieved by direct phosphorylation of the dopamine transporter protein.
      Chang, M Y, et al.
      J. Neurochem., 77: 754-61 (2001)  2001

      Show Abstract
      Immunoblotting (Western)Human11331404 11331404
      Differentiation of embryonic stem cell-derived dopaminergic neurons is enhanced by survival-promoting factors.
      Rolletschek, A, et al.
      Mech. Dev., 105: 93-104 (2001)  2001

      Show Abstract
      11429285 11429285
      Symmetrical dimer of the human dopamine transporter revealed by cross-linking Cys-306 at the extracellular end of the sixth transmembrane segment.
      Hastrup, H, et al.
      Proc. Natl. Acad. Sci. U.S.A., 98: 10055-60 (2001)  2001

      Show Abstract
      Immunoblotting (Western)Mouse11526230 11526230