324402 Anti-Dopamine D₃ Receptor Rabbit pAb

324402
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324402-100UL
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      Stocked 
      Discontinued
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.
          Catalogue Number324402
          Brand Family Calbiochem®
          References
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat brain
          PreservativeNone
          Applications
          Application ReferencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
          Key Applications Immunohistochemistry
          Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Flow Cytometry (1:100, see application references)
          Immunoblotting (1:500)
          Immunohistochemistry (1:5000)
          Application CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324402

          References

          Reference overview
          Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Alzheimer's Disease Brochure & Technical Guide
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-July-2007 RFH
          ApplicationELISA (see comments)
          Flow Cytometry (1:100, see application references)
          Immunoblotting (1:500)
          Immunohistochemistry (1:5000)
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
          IsotypeIgG
          Specieshuman, rat
          Positive controlRat brain
          FormLyophilized
          FormulationUndiluted serum.
          PreservativeNone
          CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
          Toxicity Standard Handling
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Application referencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.