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MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B

MAB5292
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5292
      ReplacesAB1791P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Dopamine D1B Receptor Antibody, clone SG4-D1B
      Alternate Names
      • Dopamine D5 Receptor
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B is validated for use in IH, WB for the detection of Dopamine D1B Receptor.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:250-1:500 on mouse brain lysate (unboiled sample)

      Immunohistochemistry

      Optimal working dilutions must be determined by the end user.

      SUGGESTED WESTERN BLOT PROTOCOL

      Solutions

      10X Transfer buffer 1 liter

      Tris 115.3 gm

      Glycine 24.2 gm

      [SDS 4 gm (may be omitted)]

      For a 1X working solution: 1 liter

      mix 700 mL water

      200 mL

      MeOH100 mL

      10X Transfer

      TBSTstock 1 liter1M

      Tris pH 7.5 10 mL

      5 M NaCl 30 mL

      10% Tween-20 5 Ml

      1. Run SDS-PAGE gel as desired.

      2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.

      3. Soak membrane in transfer buffer (see below) until ready to set up blot (>15 min) transfer.

      4. Assemble gel-transfer sandwich with the blot toward the positive electrode.

      5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.

      6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

      7. Destain with deionized water.

      8. Block with 5% non-fat milk (Marvel or Carnation) in water, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

      9. Incubation with first antibody overnight with rocking at 4°C in blocking solution. Optimal working dilutions and incubation time will need to be determined by the end user.

      10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.

      11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.

      12. Wash at least 3 x 5 min. with TBST.

      13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
      Biological Information
      ImmunogenRecombinant rat D1b Receptor, carboxy terminus.
      CloneSG4-D1B
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDopamine Receptor D1b (D5).
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2.
      Gene Symbol
      • DRD5
      • DRD1B
      • DRD1L2
      • DBDR
      • D
      • MGC10601
      Non-Reactive Species
      • Human
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
      SIZE: 477 amino acids; 52951 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.
      DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.
      SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-DOPAMINE D1b (D5) RECEPTOR MONOCLONAL ANTIBODY - 2090442 2090442
      MOUSE ANTI-DOPAMINE D1b (D5) RECEPTOR MONOCLONAL ANTIBODY - 2280862 2280862
      MOUSE ANTI-DOPAMINE D1b (D5) RECEPTOR MONOCLONAL ANTIBODY -2660715 2660715
      MOUSE ANTI-DOPAMINE D1b (D5) RECEPTOR MONOCLONAL ANTIBODY -2767244 2767244

      References

      Reference overviewPub Med ID
      Inhibition of tetrodotoxin-resistant sodium current in dorsal root ganglia neurons mediated by D1/D5 dopamine receptors.
      Galbavy, W; Safaie, E; Rebecchi, MJ; Puopolo, M
      Molecular pain 9 60 2013

      Show Abstract
      24283218 24283218
      Dopamine-induced plasticity, phospholipase D (PLD) activity and cocaine-cue behavior depend on PLD-linked metabotropic glutamate receptors in amygdala.
      Krishnan, B; Genzer, KM; Pollandt, SW; Liu, J; Gallagher, JP; Shinnick-Gallagher, P
      PloS one 6 e25639 2011

      Show Abstract
      21980514 21980514
      Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.
      Luedtke, R R, et al.
      J. Neuroimmunol., 101: 170-87 (1999) 1999

      Show Abstract
      10580800 10580800

      Data Sheet

      Title
      Anti-Dopamine D1B Receptor, clone SG4-D1B - Data Sheet