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MABE305 | Anti-Dnmt3b Antibody, clone D3B2-2C1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IP R Purified Monoclonal Antibody
      Catalogue NumberMABE305
      DescriptionAnti-Dnmt3b Antibody, clone D3B2-2C1
      Alternate Names
      • DNA (cytosine-5)-methyltransferase 3B
      • Dnmt3b
      • DNA methyltransferase HsaIIIB
      • DNA MTase HsaIIIB
      • M.HsaIIIB
      Background InformationDNA (cytosine-5)-methyltransferase 3B (Dnmt3B) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. The Dnmt3b enzyme is involved in de novo methylation and the establishment of genome-wide DNA methylation patterns during development. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.
      Product Information
      • HeLa nuclear extract
      PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Dnmt3b Antibody, clone D3B2-2C1 is a Rat Monoclonal Antibody for detection of Dnmt3b also known as DNA (cytosine-5)-methyltransferase 3B & has been validated in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt3b in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
      Biological Information
      ImmunogenLinear peptide corresponding to human Dnmt3b.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DNMT3B
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110 kDa observed. Multiple isoforms between ~80-96 kDa are known to exist. A truncated protein at ~40 kDa may be observed in some cell lysates (Ostler, K. R., et al. (2007). 26(38): 5553-5563.). Uncharacterized bands may be observed at ~20 kDa and ~34 kDa in some cell lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Dnmt3b in 10 µg of of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Dnmt3b, clone D3B2-2C1 - 2438270 2438270
      Anti-Dnmt3b, clone D3B2-2C1 - 2442558 2442558
      Anti-Dnmt3b, clone D3B2-2C1 - Q2023695 Q2023695
      Anti-Dnmt3b, clone D3B2-2C1 -2551798 2551798
      Anti-Dnmt3b, clone D3B2-2C1 -2607304 2607304
      Anti-Dnmt3b, clone D3B2-2C1 -2665806 2665806
      Anti-Dnmt3b, clone D3B2-2C1 -2820679 2820679


      Reference overviewPub Med ID
      The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1.
      Felle, Max, et al.
      Nucleic Acids Res., 39: 8355-65 (2011) 2011

      Show Abstract
      21745816 21745816

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