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MABS27 | Anti-Diglycyl Lysine (Clone GX41) Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, A, H, M WB, IP M Purified Monoclonal Antibody
      Catalogue NumberMABS27
      DescriptionAnti-Diglycyl Lysine (Clone GX41) Antibody
      Alternate Names
      • Diglycyl Lysine
      Background InformationAnti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.
      Product Information
      • BSA protein and Diglycine modified BSA
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

      Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.
      Biological Information
      ImmunogenLinear peptide corresponding to Diglycyl Lysine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.
      Species Reactivity
      • Rat
      • All
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human, mouse and rat. Broad species cross-reactivity expected based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Diglycyl Lysine
      Purification MethodProtein G
      Molecular WeightMolecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in BSA protein and Diglycine modified BSA.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Diglycyl Lysine, clone GX41 - 2462934 2462934
      Anti-Diglycyl Lysine, clone GX41 - 1990443 1990443
      Anti-Diglycyl Lysine, clone GX41 - 2080290 2080290
      Anti-Diglycyl Lysine, clone GX41 - 2117156 2117156
      Anti-Diglycyl Lysine, clone GX41 - 2211979 2211979
      Anti-Diglycyl Lysine, clone GX41 - 2227164 2227164
      Anti-Diglycyl Lysine, clone GX41 - 2246826 2246826
      Anti-Diglycyl Lysine, clone GX41 - 2298453 2298453
      Anti-Diglycyl Lysine, clone GX41 - NG1905701 NG1905701
      Anti-Diglycyl Lysine, clone GX41 - NG1943013 NG1943013
      Anti-Diglycyl Lysine, clone GX41 - NRG1855954 NRG1855954
      Anti-Diglycyl Lysine, clone GX41 -2607313 2607313
      Anti-Diglycyl Lysine, clone GX41 -2742254 2742254
      Anti-Diglycyl Lysine, clone GX41 -2764891 2764891
      Anti-Diglycyl Lysine, clone GX41 -2768868 2768868
      Anti-Diglycyl Lysine, clone GX41 -2793633 2793633
      Anti-Diglycyl Lysine, clone GX41_2842163 2842163


      Reference overviewPub Med ID
      A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles.
      Wagner, Sebastian A, et al.
      Mol. Cell Proteomics, 10: M111.013284 (2011) 2011

      Show Abstract
      21890473 21890473