MAB3118 Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, E. coli ELISA, ICC, IF, IP, WB M Ascites Monoclonal Antibody
      Catalogue NumberMAB3118
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
      Background InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

      FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
      Product Information
      • Cells transfected with FLAG-tagged fusion vector
      PresentationAscites mouse monoclonal IgG1 liquid containing no preservative.
      ApplicationDetect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence:
      A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

      A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

      A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
      Note: Does not require Ca++ for binding.

      on recombinant proteins

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin
      EpitopeEpitope tag
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityDYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.
      Species Reactivity
      • Vertebrates
      • E. coli
      Species Reactivity NoteExpected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Flag-mTor protein blot.

      Western Blot Analysis:
      1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 21179952117995
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 19591671959167
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 20322172032217
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 20646992064699
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 21959852195985
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - JC1629909JC1629909
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - LV1539274LV1539274
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - LV1583456LV1583456
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1726103NG1726103
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1736413NG1736413


      Reference overviewPub Med ID
      Multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location.
      Swedan, S; Andrews, J; Majumdar, T; Musiyenko, A; Barik, S
      Journal of virology  85  10090-100  2011

      Show Abstract
      21795342 21795342
      Respiratory syncytial virus nonstructural proteins decrease levels of multiple members of the cellular interferon pathways.
      Samer Swedan,Alla Musiyenko,Sailen Barik
      Journal of virology  83  2009

      Show Abstract Full Text Article
      19625398 19625398

      Data Sheet

      Anti-FLAG ® (DYKDDDK), (epitope tag), clone 2EL-1B11 - Data Sheet