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MAB3118 | Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

MAB3118
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, E. coli ELISA, ICC, IF, IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB3118
      ReplacesAB3250
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
      Background InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

      FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
      References
      Product Information
      FormatAscites
      Control
      • Cells transfected with FLAG-tagged fusion vector
      PresentationAscites mouse monoclonal IgG1 liquid containing no preservative.
      Applications
      ApplicationDetect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence:
      A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

      Immunoprecipitation:
      A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

      ELISA:
      A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
      Note: Does not require Ca++ for binding.

      Immunoblotting:
      on recombinant proteins

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide (Cys)-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys coupled to ovalbumin
      EpitopeEpitope tag
      Clone2EL-1B11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.
      IsotypeIgG1
      Species Reactivity
      • Vertebrates
      • E. coli
      Species Reactivity NoteExpected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Flag-mTor protein blot.

      Western Blot Analysis:
      1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2117995 2117995
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 1959167 1959167
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2032217 2032217
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2064699 2064699
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2195985 2195985
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - JC1629909 JC1629909
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - LV1539274 LV1539274
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - LV1583456 LV1583456
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1726103 NG1726103
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1736413 NG1736413
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1736414 NG1736414
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1736778 NG1736778
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1848417 NG1848417
      Anti-FLAG#174; (DYKDDDK), (epitope tag), clone 2EL-1B11 - NG1909345 NG1909345
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2392244 2392244
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2459040 2459040
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2361618 2361618
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 - 2834794 2834794
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 -2684931 2684931
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 -2709165 2709165
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 -2739416 2739416
      Anti-FLAG® (DYKDDDK), (epitope tag), clone 2EL-1B11 -2780632 2780632
      Anti-phospho-NMDAR2B (Ser1303), clone EPR1858Y, Rabbit Monoclonal - NG1739939 NG1739939

      References

      Reference overviewPub Med ID
      Multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location.
      Swedan, S; Andrews, J; Majumdar, T; Musiyenko, A; Barik, S
      Journal of virology 85 10090-100 2011

      Show Abstract
      21795342 21795342
      Respiratory syncytial virus nonstructural proteins decrease levels of multiple members of the cellular interferon pathways.
      Samer Swedan,Alla Musiyenko,Sailen Barik
      Journal of virology 83 2009

      Show Abstract Full Text Article
      19625398 19625398

      Data Sheet

      Title
      Anti-FLAG ® (DYKDDDK), (epitope tag), clone 2EL-1B11 - Data Sheet