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NA57 | Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

NA57
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      NA57-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
          Catalogue NumberNA57
          Brand Family Calbiochem®
          SynonymsAnti-DNA Dependent Protein Kinase
          Application Data
          Detection of human DNA-PK by immunohistochemistry. Sample: Human tonsil tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-DNA-PK (Ab-2) Mouse mAb (18-2) (Cat. No. NA57) (1:100). Detection: fluorescence.

          Detection of human DNA-PK by immunoblotting. Sample: Human DNA-PK. Primary antibody: Anti-DNA-PK (Ab-2) Mouse mAb (18-2) (Cat. No. NA57) (1 µg/ml). Detection: chemiluminescence.
          References
          References<→EMD:if(output-is PDS)→> Kharbanda, S., et al. 1997. Nature 386, 732.
          McConnell, K.R., et al. 1997 J. Immunol. 158, 2083-2089.
          Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
          Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
          Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
          Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
          Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
          Connelly, M.A., et al. 1996. Gene 175, 271.
          Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
          Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
          Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
          Song, Q., et al. 1996. EMBO J. 15, 3238.
          Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
          Blunt, T., et al. 1995. Cell 80, 813.
          Hartley, K.O., et al. 1995. Cell 82, 849.
          Kirchgessner, C.U., et al. 1995. Science 267, 1178.
          Lees-Miller, S.P., et al. 1995. Science 267, 1183.
          Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
          Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
          Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131. <→EMD:endif()→>
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
          Positive controlA-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Carter, T., et al. 1990. Mol. Cell. Biol. 10, 6460.
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Neutralization Studies
          Paraffin Sections
          Application NotesFrozen Sections (1:50-1:100)
          Immunoblotting (1:250-1:500)
          Immunofluorescence (1:50-1:100)
          Immunoprecipitation (10 µl/mg protein lysate)
          Neutralization Studies (see comments)
          Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
          Application CommentsThis antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenDNA-PK purified from HeLa cells
          ImmunogenHuman
          Epitopewithin amino acids 1-2713 of DNA-PK
          Clone18-2
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          NA57

          References

          Reference overview
          <→EMD:if(output-is PDS)→> Kharbanda, S., et al. 1997. Nature 386, 732.
          McConnell, K.R., et al. 1997 J. Immunol. 158, 2083-2089.
          Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
          Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
          Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
          Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
          Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
          Connelly, M.A., et al. 1996. Gene 175, 271.
          Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
          Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
          Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
          Song, Q., et al. 1996. EMBO J. 15, 3238.
          Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
          Blunt, T., et al. 1995. Cell 80, 813.
          Hartley, K.O., et al. 1995. Cell 82, 849.
          Kirchgessner, C.U., et al. 1995. Science 267, 1178.
          Lees-Miller, S.P., et al. 1995. Science 267, 1183.
          Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
          Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
          Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131. <→EMD:endif()→>

          Brochure

          Title
          Biologics 33.1 ( 1.37 MB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-September-2007 RFH
          SynonymsAnti-DNA Dependent Protein Kinase
          ApplicationFrozen Sections (1:50-1:100)
          Immunoblotting (1:250-1:500)
          Immunofluorescence (1:50-1:100)
          Immunoprecipitation (10 µl/mg protein lysate)
          Neutralization Studies (see comments)
          Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
          Application Data
          Detection of human DNA-PK by immunohistochemistry. Sample: Human tonsil tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-DNA-PK (Ab-2) Mouse mAb (18-2) (Cat. No. NA57) (1:100). Detection: fluorescence.

          Detection of human DNA-PK by immunoblotting. Sample: Human DNA-PK. Primary antibody: Anti-DNA-PK (Ab-2) Mouse mAb (18-2) (Cat. No. NA57) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
          BackgroundDNA Dependent Protein Kinase, DNA-PK, is a nuclear serine/threonine protein kinase composed of two subunits, a ~470 kDa catalytic subunit, DNA-PKcs, and the Ku autoantigen, the heterodimeric DNA binding protein p70/p80. DNA-PK has been shown to phosphorylate a number of key proteins in vitro including p53, Sp1, myc, RPA, and RNA Pol I. DNA-PK activity requires association of DNA-PKcs with Ku, DNA binding, and the presence of DNA ends. Mutations in DNA-PKcs contribute to murine SCID phenotypes which leads to inefficient V(D)J recombination in developing T & B lymphocytes. Loss of DNA-PK activity in fibroblasts or lymphocytes also confers on cells hypersensitivity to killing by ionizing radiation. These two phenotypes implicate DNA-PK in the repair of DNA double strand breaks in all cells and V(D)J recombination in lymphocytes. The 4127 amino acid DNA-PKcs protein shares a high degree of similarity with the ATM family of kinases, including ATM, ATR, and FRAP in humans and MEC1 and TEL1 in yeast. The catalytic domain of DNA-PKcs also shares homology with the PI3K family of lipid kinases and DNA-PK is inhibitable by wortmanin, a lipid kinase inhibitor. While the exact role for DNA-PK in cell cycle regulation is unclear, the protein may be important for suppression of apoptosis since DNA-PKcs is targeted for proteolysis during apoptosis, which leads to loss of catalytic activity. DNA-PK can also be inactivated through phosphorylation, possibly by c-abl and by autophosphorylation. DNA-PKcs is expressed in all cells and a DNA-PK activity is observed in all higher eukaryotes examined. The protein level of DNA-PKcs in human cells is very high, 50 to 100x that observed in murine cells.
          HostMouse
          Immunogen speciesHuman
          ImmunogenDNA-PK purified from HeLa cells
          Epitopewithin amino acids 1-2713 of DNA-PK
          Clone18-2
          IsotypeIgG₁
          Specieshuman
          Positive controlA-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
          FormLiquid
          FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          References<→EMD:if(output-is PDS)→> Kharbanda, S., et al. 1997. Nature 386, 732.
          McConnell, K.R., et al. 1997 J. Immunol. 158, 2083-2089.
          Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
          Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
          Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
          Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
          Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
          Connelly, M.A., et al. 1996. Gene 175, 271.
          Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
          Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
          Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
          Song, Q., et al. 1996. EMBO J. 15, 3238.
          Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
          Blunt, T., et al. 1995. Cell 80, 813.
          Hartley, K.O., et al. 1995. Cell 82, 849.
          Kirchgessner, C.U., et al. 1995. Science 267, 1178.
          Lees-Miller, S.P., et al. 1995. Science 267, 1183.
          Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
          Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
          Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131. <→EMD:endif()→>
          Application referencesOriginal Clone Carter, T., et al. 1990. Mol. Cell. Biol. 10, 6460.