MAB3864 Anti-DNA/Histone H1 Antibody

MAB3864
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, FC, ICC, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3864
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DNA/Histone H1 Antibody
      References
      Product Information
      FormatPurified
      PresentationProtein A purified culture supernatant. Liquid in PBS
      Applications
      ApplicationAnti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

      Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

      Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

      ELISA, direct and indirect

      Optimal working dilutions must be determined by end user.

      STAINING PATTERN:

      Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
      Biological Information
      ImmunogenHuman Raji cell nuclei
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.
      IsotypeIgG2a
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1E
      • H1.4
      • dJ221C16.5
      • H1F4
      • MGC116819
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 219 amino acids; 21865 Da
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
      MISCELLANEOUS: This variant accounts for 60% of histone H1.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-DNA/Histone H1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DNA/Histone H1 Antibody Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-DNA/HISTONE 1 - 32694493269449
      MOUSE ANTI-DNA/HISTONE 1 -25523562552356
      MOUSE ANTI-DNA/HISTONE 1 -26984552698455
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY2907700
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY3018881
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY - 23877782387778
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY - 24531822453182
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY - 20322192032219
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY - 20487632048763
      MOUSE ANTI-DNA/HISTONE 1 MONOCLONAL ANTIBODY - 20684262068426

      References

      Reference overviewPub Med ID
      Neutrophil Responses to Sterile Implant Materials.
      Jhunjhunwala, S; Aresta-DaSilva, S; Tang, K; Alvarez, D; Webber, MJ; Tang, BC; Lavin, DM; Veiseh, O; Doloff, JC; Bose, S; Vegas, A; Ma, M; Sahay, G; Chiu, A; Bader, A; Langan, E; Siebert, S; Li, J; Greiner, DL; Newburger, PE; von Andrian, UH; Langer, R; Anderson, DG
      PloS one  10  e0137550  2015

      Show Abstract
      26355958 26355958
      Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones.
      Saffarzadeh, Mona, et al.
      PLoS ONE, 7: e32366 (2012)  2012

      Show Abstract
      22389696 22389696
      Pharmacokinetic characteristics and biodistribution of radioiodinated chimeric TNT-1, -2, and -3 monoclonal antibodies after chemical modification with biotin.
      Khawli, Leslie A, et al.
      Cancer Biother. Radiopharm., 17: 359-70 (2002)  2002

      Show Abstract
      12396700 12396700
      Cell based radioimmunoassays to quantitate the immunoreactivity of TNT monoclonal antibodies directed against intracellular antigens.
      Gaffar, S A, et al.
      Journal of immunoassay, 12: 1-14 (1991)  1991

      Show Abstract
      2040706 2040706
      A comparative autoradiographic study demonstrating differential intratumor localization of monoclonal antibodies to cell surface (Lym-1) and intracellular (TNT-1) antigens.
      Chen, F M, et al.
      J. Nucl. Med., 31: 1059-66 (1990)  1990

      Show Abstract
      2348235 2348235

      Data Sheet

      Title
      MOUSE ANTI-DNA/HISTONE 1

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies