A part of MilliporeSigma

MAB030 | Anti-DNA Antibody, double stranded, clone BV16-13

MAB030
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, RIA, ICC M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMAB030
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DNA Antibody, double stranded, clone BV16-13
      References
      Product Information
      FormatCulture Supernatant
      PresentationUnpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
      Applications
      ApplicationDetect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
      Key Applications
      • ELISA
      • Radioimmunoassay
      • Immunocytochemistry
      Application NotesEIA/RIA

      Immunocytochemistry

      Optimal working dilutions must be determined by end user.
      Biological Information
      Epitopedouble stranded, 28% reactive with single stranded
      CloneBV16-13
      HostMouse
      SpecificityDouble stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

      BINDING ASSAY:

      Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



      ANTIGEN BINDING

      PROFILE: Relative Binding To:



      Natural DNA Synthetic homopolymers

      dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

      1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



      Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



      a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-DOUBLE STRANDED DNA - 2827151 2827151
      MOUSE ANTI-DOUBLE STRANDED DNA -2557656 2557656
      MOUSE ANTI-DOUBLE STRANDED DNA -2593896 2593896
      MOUSE ANTI-DOUBLE STRANDED DNA -2628819 2628819
      MOUSE ANTI-DOUBLE STRANDED DNA -2669936 2669936
      MOUSE ANTI-DOUBLE STRANDED DNA -2694550 2694550
      MOUSE ANTI-DOUBLE STRANDED DNA -2800169 2800169
      MOUSE ANTI-DOUBLE STRANDED DNA MONOCLONAL ANTIBODY - 1979941 1979941
      MOUSE ANTI-DOUBLE STRANDED DNA MONOCLONAL ANTIBODY - 2061443 2061443
      MOUSE ANTI-DOUBLE STRANDED DNA MONOCLONAL ANTIBODY - 2273997 2273997
      MOUSE ANTI-DOUBLE STRANDED DNA MONOCLONAL ANTIBODY - 2532257 2532257

      References

      Reference overviewPub Med ID
      Noncanonical autophagy is required for type I interferon secretion in response to DNA-immune complexes.
      Henault, J; Martinez, J; Riggs, JM; Tian, J; Mehta, P; Clarke, L; Sasai, M; Latz, E; Brinkmann, MM; Iwasaki, A; Coyle, AJ; Kolbeck, R; Green, DR; Sanjuan, MA
      Immunity 37 986-97 2012

      Show Abstract
      23219390 23219390
      Progression of lupus-like disease drives the appearance of complement-activating IgG antibodies in MRL/lpr mice.
      Papp, K; Végh, P; Tchorbanov, A; Vassilev, T; Erdei, A; Prechl, J
      Rheumatology (Oxford, England) 49 2273-80 2010

      Show Abstract
      20736228 20736228
      Preparation and immunolabeling of Caenorhabditis elegans.
      Crittenden S, Kimble J
      CSH protocols 2009 pdb.prot5216 2009

      20147168 20147168
      Visualization of RecA filaments and DNA by fluorescence microscopy.
      Taro Nishinaka, Yuko Doi, Makiko Hashimoto, Reiko Hara, Takehiko Shibata, Yoshie Harada, Kazuhiko Kinosita, Hiroyuki Noji, Eiji Yashima
      Journal of biochemistry 141 147-56 2007

      Show Abstract
      17202195 17202195
      Confocal methods for Caenorhabditis elegans.
      S L Crittenden, J Kimble
      Methods in molecular biology (Clifton, N.J.) 122 141-51 1999

      10231789 10231789
      Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4.
      W B Raich, A N Moran, J H Rothman, J Hardin
      Molecular biology of the cell 9 2037-49 1998

      Show Abstract Full Text Article
      9693365 9693365
      The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos.
      A R Skop, J G White, A R Skop, J G White
      Current biology : CB 8 1110-6 1998

      Show Abstract
      9778526 9778526
      Detection and isolation of lectin-transfected COS cells based on cell adhesion to immobilized glycosphingolipids.
      L J Yang, C B Zeller, R L Schnaar
      Analytical biochemistry 236 161-7 1996

      Show Abstract
      8619482 8619482
      Variable region primary structures of monoclonal anti-DNA autoantibodies from NZB/NZW F1 mice.
      Smith, R G and Voss, E W
      Mol. Immunol., 27: 463-70 (1990) 1990

      Show Abstract
      2114528 2114528
      Base specificity and idiotypy of anti-DNA autoantibodies reactive with synthetic nucleic acids.
      Ballard, D W and Voss, E W
      J. Immunol., 135: 3372-80 (1985) 1985

      Show Abstract
      3876384 3876384

      Data Sheet

      Title
      MOUSE ANTI-DOUBLE STRANDED DNA