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MAB3232 | Anti-Cytokeratin 14 Antibody, clone RCK107

MAB3232
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, Po, R FC, WB, ICC, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3232
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 14 Antibody, clone RCK107
      Background InformationCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
      References
      Product Information
      FormatPurified
      PresentationLiquid in buffer with 0.1% sodium azide.
      Applications
      ApplicationDetect Cytokeratin 14 using this Anti-Cytokeratin 14 Antibody, clone RCK107 validated for use in FC, WB, IC, IH.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot

      Immunohistochemistry on frozen tissue sections.

      Immunocytochemistry

      Flow cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytoskeletal preparation of TR146 epithelial cells.
      CloneRCK107
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityCytokeratin 14. MAB3232 reacts with basal cell compartments of stratified and combined epithelia.Reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands.
      IsotypeIgG3
      Species Reactivity
      • Canine
      • Human
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
      Gene Symbol
      • KRT14
      • Cytokeratin-14
      • NFJ
      • CK-14
      • CK14
      • EBS3
      • EBS4
      • K14
      • Keratin-14
      UniProt Number
      UniProt SummarySIZE: 472 amino acids; 51622 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD.
      TISSUE SPECIFICITY: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
      DISEASE: SwissProt: P02533 # Defects in KRT14 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 601001]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%). Blistering occurs within the basal cell layer and is further divided into three subgroups. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal- epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT14 are the cause of Naegeli-Franceschetti- Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects. & Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistant reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
      SIMILARITY: SwissProt: P02533 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2120003 2120003
      MOUSE ANTI-CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2366515 2366515
      MOUSE ANTI-CYTOKERATIN 14 -2714562 2714562
      MOUSE ANTI-CYTOKERATIN 14 -2788903 2788903
      MOUSE ANTI-CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2025320 2025320
      MOUSE ANTI-CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2153003 2153003

      References

      Reference overviewPub Med ID
      Ryanodine receptors are expressed in epidermal keratinocytes and associated with keratinocyte differentiation and epidermal permeability barrier homeostasis.
      Denda, S; Kumamoto, J; Takei, K; Tsutsumi, M; Aoki, H; Denda, M
      The Journal of investigative dermatology 132 69-75 2012

      Show Abstract
      21881589 21881589
      Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells.
      Shimura, T; Noma, N; Oikawa, T; Ochiai, Y; Kakuda, S; Kuwahara, Y; Takai, Y; Takahashi, A; Fukumoto, M
      Oncogenesis 1 e12 2012

      Show Abstract
      23552696 23552696
      Real-time monitoring of membrane cholesterol reveals new insights into epidermal differentiation.
      Florian Spörl,Minetta Wunderskirchner,Oliver Ullrich,Gerrit Bömke,Ute Breitenbach,Thomas Blatt,Horst Wenck,Klaus-Peter Wittern,Annika Schrader
      The Journal of investigative dermatology 130 2010

      Show Abstract
      20043016 20043016
      Dual role for Id2 in chemical carcinogen-induced skin tumorigenesis.
      Atsushi Tokuriki,Tomonori Iyoda,Kayo Inaba,Koichi Ikuta,Shinji Fujimoto,Masanobu Kumakiri,Yoshifumi Yokota
      Carcinogenesis 30 2009

      Show Abstract
      19587095 19587095
      Differentiation of tracheal basal cells to ciliated cells and tissue reconstruction on the synthesized basement membrane substratum in vitro.
      Takeshi Hosokawa,Tomoko Betsuyaku,Masaharu Nishimura,Akiko Furuyama,Kazuko Katagiri,Katsumi Mochitate
      Connective tissue research 48 2007

      Show Abstract
      17364662 17364662
      Evidence for specific TRPM8 expression in human prostate secretory epithelial cells: functional androgen receptor requirement.
      Bidaux, G; Roudbaraki, M; Merle, C; Crépin, A; Delcourt, P; Slomianny, C; Thebault, S; Bonnal, JL; Benahmed, M; Cabon, F; Mauroy, B; Prevarskaya, N
      Endocrine-related cancer 12 367-82 2005

      Show Abstract
      15947109 15947109
      Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18.
      Wetzels, R H, et al.
      Histopathology, 20: 295-303 (1992) 1992

      Show Abstract
      1374358 1374358

      Data Sheet

      Title
      MOUSE ANTI-CYTOKERATIN 14 MONOCLONAL ANTIBODY - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies