A part of MilliporeSigma

AB1255 | Anti-Cytochrome P450 Enzyme CYP1A1 and CYP1A2 Antibody

AB1255
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R ELISA, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1255
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytochrome P450 Enzyme CYP1A1 and CYP1A2 Antibody
      References
      Product Information
      FormatSerum
      Control
      • POSITIVE CONTROL: rat hepatic microsomal fraction.
      PresentationRabbit antiserum. Liquid. Contains no preservative.
      Applications
      ApplicationDetect Cytochrome P450 Enzyme CYP1A1 & CYP1A2 using this Anti-Cytochrome P450 Enzyme CYP1A1 & CYP1A2 Antibody validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: useful for the simultaneous detection of rat CYP1A1 (57 kDa) and CYP1A2 (54 kDa), which can be separated electrophoretically.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide
      HostRabbit
      SpecificityReacts with rat cytochrome P450 enzyme CYP1A1 and CYP1A2 on Western blots of hepatic microsomal fractions. No cross-reactivity with other cytochrome P450 enzymes.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
      Gene Symbol
      • CYP1A1
      • CP11
      • P1-450
      • CYPIA1
      • P450-C
      • CYP1
      • P450DX
      • P450-P1
      • AHRR
      • AHH
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05177 # Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen.
      SIZE: 515 amino acids; 58294 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
      TISSUE SPECIFICITY: Liver.
      SIMILARITY: SwissProt: P05177 ## Belongs to the cytochrome P450 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-RAT CYTOCHROME P450 CYP1A1 AND CYP1A2 - 2494576 2494576
      RABBIT ANTI-RAT CYTOCHROME P450 CYP1A1 AND CYP1A2 POLYCLONAL ANTIBODY - 1994883 1994883
      RABBIT ANTI-RAT CYTOCHROME P450 CYP1A1 AND CYP1A2 POLYCLONAL ANTIBODY - 2316452 2316452

      References

      Reference overviewPub Med ID
      A role of Wnt/beta-catenin signals in hepatic fate specification of human umbilical cord blood-derived mesenchymal stem cells.
      Yoshida, Y; Shimomura, T; Sakabe, T; Ishii, K; Gonda, K; Matsuoka, S; Watanabe, Y; Takubo, K; Tsuchiya, H; Hoshikawa, Y; Kurimasa, A; Hisatome, I; Uyama, T; Terai, M; Umezawa, A; Shiota, G
      American journal of physiology. Gastrointestinal and liver physiology 293 G1089-98 2007

      Show Abstract
      17884977 17884977
      Identification of the epitope of an anti-peptide antibody which binds to CYP1A2 in many species including man
      Edwards, R J, et al
      Biochem Pharmacol, 46:213-20 (1993) 1993

      7688509 7688509
      Synthetic peptide antigens elicit monoclonal and polyclonal antibodies to cytochrome P450 IA2.
      Myers, M J, et al.
      Biochem. Biophys. Res. Commun., 169: 171-6 (1990) 1990

      Show Abstract
      2350341 2350341

      Data Sheet

      Title
      RABBIT ANTI-RAT CYTOCHROME P450 CYP1A1 AND CYP1A2