Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||ICC, IP, WB||M||Purified||Monoclonal Antibody|
|Description||Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4|
|Presentation||Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at 2-8°C in undiluted aliquots up to 6 months.|
|Material Size||100 µg|
|Reference overview||Pub Med ID|
|Antagonistic regulation of cystic fibrosis transmembrane conductance regulator cell surface expression by protein kinases WNK4 and spleen tyrosine kinase. |
Mendes, AI; Matos, P; Moniz, S; Luz, S; Amaral, MD; Farinha, CM; Jordan, P
Molecular and cellular biology 31 4076-86 2011
Members of the WNK (with-no-lysine [K]) subfamily of protein kinases regulate various ion channels involved in sodium, potassium, and chloride homeostasis by either inducing their phosphorylation or regulating the number of channel proteins expressed at the cell surface. Here, we describe findings demonstrating that the cell surface expression of the cystic fibrosis transmembrane conductance regulator (CFTR) is also regulated by WNK4 in mammalian cells. This effect of WNK4 is independent of the presence of kinase and involves interaction with and inhibition of spleen tyrosine kinase (Syk), which phosphorylates Tyr512 in the first nucleotide-binding domain 1 (NBD1) of CFTR. Transfection of catalytically active Syk into CFTR-expressing baby hamster kidney cells reduces the cell surface expression of CFTR, whereas that of WNK4 promotes it. This is shown by biotinylation of cell surface proteins, immunofluorescence microscopy, and functional efflux assays. Mutation of Tyr512 to either glutamic acid or phenylalanine is sufficient to alter CFTR surface levels. In human airway epithelial cells, downregulation of endogenous Syk and WNK4 confirms their roles as physiologic regulators of CFTR surface expression. Together, our results show that Tyr512 phosphorylation is a novel signal regulating the prevalence of CFTR at the cell surface and that WNK4 and Syk perform an antagonistic role in this process.
|Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution. |
Jiang H, Ramos AA, Yao X
Anal Chem 82 336-42. 2010
Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new CF therapeutics and treatments, which requires a sensitive and precise method for quantifying apical plasma membrane CFTR. We report the first method of liquid chromatography-tandem mass spectrometry for quantifying endogenous and overexpressed CFTR in HT29 and BHK cells. For low level of endogenous CFTR from HT29, the target protein in the cell lysate was enriched by immunoprecipitation using anti-CFTR antibody MAB3484 or M3A7. For overexpressed CFTR from BHK, the cell lysate prepared by differential detergent fractionation or surface biotinylation was used directly without immunoprecipitation. Proteins in the enriched CFTR preparations or cell lysates were digested with proteases, and a surrogate marker peptide designated as CFTR01 (NSILTETLHR) was successfully quantified using the method of multiple reaction monitoring and stable isotope dilution with an (18)O-labeled reference peptide (CFTR01-(18)O(4)) as the internal standard. CFTR quantified in this work ranged from a few tens of picograms to low nanograms per million of cells.
|Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways. |
Heather Davidson, Gerry McLachlan, Abigail Wilson, A Christopher Boyd, Ann Doherty, Gordon MacGregor, Lee Davies, Hazel A Painter, Rebecca Coles, Stephen C Hyde, Deborah R Gill, Margarida D Amaral, David D S Collie, David J Porteous, Deborah Penque
American journal of respiratory cell and molecular biology 35 72-83 2006
A panel of 11 human cystic fibrosis transmembrane conductance regulator (hCFTR) antibodies were tested in ovine nasal, tracheal, and bronchial epithelial brushings. Two of these, G449 (polyclonal) and MATG1104 (monoclonal), recognized hCFTR but did not cross react with endogenous sheep CFTR. This specificity allows immunologic detection of hCFTR expressed in gene transfer studies in sheep against the background of endogenous ovine CFTR, thus enhancing the value of the sheep as a model animal in which to study CFTR gene transfer. Studies on mixed populations of human and sheep nasal epithelial cells showed that detection of hCFTR by these two antibodies was possible even at the lowest proportion of human cells (1:100). The hCFTR gene was delivered in vivo by local instillation using polyethylenimine-mediated gene transfer to the ventral surface of the ovine trachea and hCFTR mRNA and protein levels scored in a blinded fashion. Despite abundant hCFTR mRNA expression, the number of cells expressing hCFTR protein detectable by G449 was low (approximately 0.006-0.05%). Immunohistochemistry for hCFTR in animals treated by whole-lung aerosol demonstrated positive cells in sections of tracheal epithelium and in distal conducting airways. The strategic use of hCFTR-specific antibodies supports the utility of the normal sheep as a model for hCFTR gene transfer studies.
|CFTR localization in native airway cells and cell lines expressing wild-type or F508del-CFTR by a panel of different antibodies |
Carvalho-Oliveira, Isabel, et al
J Histochem Cytochem, 52:193-203 (2004) 2004
|A comparison of 14 antibodies for the biochemical detection of the cystic fibrosis transmembrane conductance regulator protein |
Farinha, Carlos M, et al
Mol Cell Probes, 18:235-42 (2004) 2004
|Antibodies for CFTR studies |
Mendes, Filipa, et al
J Cyst Fibros, 3 Suppl 2:69-72 (2004) 2004
|Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator. |
Zhang, H; Peters, KW; Sun, F; Marino, CR; Lang, J; Burgoyne, RD; Frizzell, RA
The Journal of biological chemistry 277 28948-58 2002
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel whose phosphorylation regulates both channel gating and its trafficking at the plasma membrane. Cysteine string proteins (Csps) are J-domain-containing, membrane-associated proteins that have been functionally implicated in regulated exocytosis. Therefore, we evaluated the possibility that Csp is involved in regulated CFTR trafficking. We found Csp expressed in mammalian epithelial cell lines, several of which express CFTR. In Calu-3 airway cells, immunofluorescence colocalized Csp with calnexin in the endoplasmic reticulum and with CFTR at the apical membrane domain. CFTR coprecipitated with Csp from Calu-3 cell lysates. Csp associated with both core-glycosylated immature and fully glycosylated mature CFTRs (bands B and C); however, in relation to the endogenous levels of the B and C bands expressed in Calu-3 cells, the Csp interaction with band B predominated. In vitro protein binding assays detected physical interactions of both mammalian Csp isoforms with the CFTR R-domain and the N terminus, having submicromolar affinities. In Xenopus oocytes expressing CFTR, Csp overexpression decreased the chloride current and membrane capacitance increases evoked by cAMP stimulation and decreased the levels of CFTR protein detected by immunoblot. In mammalian cells, the steady-state expression of CFTR band C was eliminated, and pulse-chase studies showed that Csp coexpression blocked the conversion of immature to mature CFTR and stabilized band B. These results demonstrate a primary role for Csp in CFTR protein maturation. The physical interaction of this Hsc70-binding protein with immature CFTR, its localization in the endoplasmic reticulum, and the decrease in production of mature CFTR observed during Csp overexpression reflect a role for Csp in CFTR biogenesis. The documented role of Csp in regulated exocytosis, its interaction with mature CFTR, and its coexpression with CFTR at the apical membrane domain of epithelial cells may reflect also a role for Csp in regulated CFTR trafficking at the plasma membrane.
|Lacking CD56 expression in a relapsing cutaneous blastic plasmacytoid dendritic cell neoplasm after allogeneic bone marrow transplantation: FISH analysis revealed loss of 11q. |
Mitteldorf C, Bertsch HP, Baumgart M, Haase D, Wulf G, Schön MP, Rosenwald A, Neumann C, Kaune KM.
Journal of the European Academy of Dermatology and Venereology : JEADV 2001
|MOUSE ANTI-CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (CFTR) MONOCLONAL ANTIBODY|