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MAB3100 | Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

MAB3100
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Rb ICC, IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2
      Alternate Names
      • Cx45
      • Gap Junction gamma-1 protein
      • Gap Junction alpha-7 protein
      • Cx-45
      • GJA7 protein
      • GJC1 protein
      Background InformationConnexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.
      References
      Product Information
      FormatPurified
      Control
      • Cardiac tissue, particularly sinoatrial tissues.
      PresentationLiquid at 1 mg/mL in 0.02M PB with 0.25M NaCl,pH=7.6 containing 0.1% sodium azide as a preservative.
      Applications
      ApplicationAnti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunblotting (~46 kDa): 0.5 -1 µg/mL with ECL detection. Samples should be prepared with proteinase inhibitors and kept cold for best results. Alkali extraction methods can be used to enrich for connexin protein fractions. Briefly, 1 ml of NaHCO3 containing protease and phosphatase inhibitors and 22 µl of 1 M NaOH were added to frozen cell pellets. This cell suspension was sonicated for 30 s and incubated on ice for 50 min. Then, the homogenate was centrifuged for 30 min at 30,000 X g at 4°C. The supernatant was discarded, and the pellet was resuspended in sample buffer. {Elenes, S et al 2001}. 8% SDS_PAGE gels and PVDF membranes are suggested for greatest clarity.

      Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.

      Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.
      Epitopenear C-terminus, cytoplasmic
      Clone8A11.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityClone 8A11.2 is highly specific for Connexin 45 protein with no cross reactivity as analyzed by western blotting to any other connexin family member.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Rabbit
      Species Reactivity NoteHuman, rat (Sorensen, et al 2008), mouse, chicken (Elenes, et al (2001), rabbit (Chaytor, et al 2005), and dog (Wang, et al (2001), others species not tested. Also confirmed to work on rat C6 cells.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ENSG00000182963
      • Cx45
      • Connexin-45
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36383 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
      SIZE: 396 amino acids; 45483 Da
      SUBUNIT: A connexon is composed of a hexamer of connexins.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
      SIMILARITY: SwissProt: P36383 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2° to 8°C in undiluted aliquots for up to 12 months fromd date of receipt. Prevent multiple warming and coolings. Aliquot in tightly closed tubes to prevent loss.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] 2470966
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] MONOCLONAL ANTIBODY -
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] MONOCLONAL ANTIBODY - 2110494 2110494
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] MONOCLONAL ANTIBODY - 2393904 2393904
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] MONOCLONAL ANTIBODY - 2273823 2273823
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45] MONOCLONAL ANTIBODY -2576300 2576300
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45], MONOCLONAL ANTIBODY 2841531
      MOUSE ANTI-HUMAN CONNEXIN 45 [CX45]_2840175 2840175

      References | 12 Available | See All References

      Reference overviewApplicationPub Med ID
      Arachnoid Cells on Culture Plates and Collagen Scaffolds: Phenotype and Transport Properties.
      Lam CH, Hansen E, Hubel A
      Tissue Eng Part A 2011

      Show Abstract
      21306279 21306279
      Human cardiac gap-junction coupling: effects of antiarrhythmic peptide AAP10.
      Anja Hagen,Anna Dietze,Stefan Dhein
      Cardiovascular research 83 2009

      Show Abstract
      19176598 19176598
      Connexin mimetic peptides fail to inhibit vascular conducted calcium responses in renal arterioles.
      Sorensen, CM; Salomonsson, M; Braunstein, TH; Nielsen, MS; Holstein-Rathlou, NH
      American journal of physiology. Regulatory, integrative and comparative physiology 295 R840-7 2008

      Show Abstract
      Immunofluorescence18614765 18614765
      Inhibition of connexin 43 expression and function in cultured rat dental pulp cells by antisense oligonucleotide.
      Chung, Chul-Kyun, et al.
      Cell Tissue Res., 329: 295-300 (2007) 2007

      17450382 17450382
      Role of gap junctions in spontaneous activity of the rat bladder.
      Ikeda, Y, et al.
      Am. J. Physiol. Renal Physiol., 293: F1018-25 (2007) 2007

      17581924 17581924
      Mechanical loading stimulates expression of connexin 43 in alveolar bone cells in the tooth movement model.
      Gluhak-Heinrich, J; Gu, S; Pavlin, D; Jiang, JX
      Cell communication & adhesion 13 115-25 2006

      Show Abstract Full Text Article
      16613785 16613785
      Connexin-mimetic peptides dissociate electrotonic EDHF-type signalling via myoendothelial and smooth muscle gap junctions in the rabbit iliac artery.
      Chaytor, Andrew T, et al.
      Br. J. Pharmacol., 144: 108-14 (2005) 2005

      Show Abstract
      15644874 15644874
      Dynamic model for ventricular junctional conductance during the cardiac action potential.
      Lin, X; Gemel, J; Beyer, EC; Veenstra, RD
      American journal of physiology. Heart and circulatory physiology 288 H1113-23 2005

      Show Abstract
      15513960 15513960
      Ageing-related changes of connexins and conduction within the sinoatrial node.
      Jones, Sandra A, et al.
      J. Physiol. (Lond.), 560: 429-37 (2004) 2004

      Show Abstract
      15308686 15308686
      Connexin immunoreactivity in glial cells of the rat retina.
      Zahs, Kathleen R, et al.
      J. Comp. Neurol., 455: 531-46 (2003) 2003

      Show Abstract
      12508325 12508325

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies