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MABF972 | Anti-Complement C3b/iC3b Antibody, clone 3E7, neutralizing

MABF972
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Pm FC, NEUT, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABF972
      DescriptionAnti-Complement C3b/iC3b Antibody, clone 3E7, neutralizing
      Alternate Names
      • Complement C3
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      • Complement C3 beta chain
      • C3-beta-c
      • C3bc
      • Complement C3 alpha chain
      • C3a anaphylatoxin
      • Acylation stimulating protein
      • ASP
      • C3adesArg
      • Complement C3b alpha' chain
      • Complement C3c alpha' chain fragment 1
      • Complement C3dg fragment
      • Complement C3g fragment
      • Complement C3d fragment
      • Complement C3f fragment
      • Complement C3c alpha' chain fragment 2
      • Complement C3b/iC3b
      • Complement C3
      Background InformationComplement C3 (UniProt P01024; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID 718) in human. C3 is initially translated with an N-terminal 22-amino acid signal peptide sequence, which is then removed to produce the 1641-amino acid mature C3. It plays a central role in the activation of the complement system. Its activation is required for the activation of both classical and alternative pathway of complement (CPC and APC, respectively). C3 is cleaved into C3a and C3b during CPC activation by the C3-convertase C4b2a composed of the activated C4 and C2. In APC, C3 is cleaved by the C3-convertase C3bBb composed C3b and the activated form of factor B (Bb). C3b serves as an opsonizing agent, and can be further cleaved by Factor I into C3c and C3d. iC3b is a proteolytically inactive C3b fragment that still opsonizes target microbes or cells, but cannot further amplify/activate the complement cascade through APC. iC3b can be further cleaved to C3dg, and finally to C3d. Unregulated activation of APC can result in paroxysmal nocturnal hemoglobinuria (PNH) that is characterized by chronic intravascular hemolysis. Clinical C5-neutralizing mAb treatment prevents the formation of cytolytic membrane attack complex (MAC) of complement, but does not block APC activation. Consequently, PNH patients are left with immune-mediated hemolytic anemia and their erythrocytes become opsonized with complement C3. Monoclonal antibodies (mAbs) against C3b/iC3b are useful for monitoring and studying C3b/iC3b deposit on PNH blood cells and mAbs with neutralizing activities are useful tools for studying C3-mediated CPC and APC.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
      Applications
      ApplicationThis mouse monoclonal Anti-Complement C3b/iC3b Antibody, clone 3E7, Cat. No. MABF972 is a neutralizing antibody validated for use in Flow Cytometry, for the detection of C3b.
      Key Applications
      • Flow Cytometry
      • Neutralizing
      • Immunocytochemistry
      Application NotesNeutralizing Analysis: This clone has been shown to prevent alternative pathway of complement (APC) activation-mediated hemolysis of IgM-opsinized paroxysmal nocturnal hemoglobinuria (PNH) erthyrocytes (Lindorfer, M.A., et al. (2010). Blood. 115(11):2283-2291).
      Flow Cytometry Analysis: A presentive lot detected C3b/iC3b deposit on anti-CD20 mAb Rituximab-opsinized human peripheral blood ARH-77 & Raji B cells, as well as Rituximab-opsinized CD20+ cells freshly isolated from monkey blood (Kennedy, A.D., et al. (2003). Blood. 101(3):1071-1079).
      Immunocytochemistry Analysis: A presentive lot detected C3b/iC3b deposit on anti-CD20 mAb Rituximab-opsinized human peripheral blood ARH-77 & Raji B cells, as well as Rituximab-opsinized CD20+ cells freshly isolated from monkey blood (Kennedy, A.D., et al. (2003). Blood. 101(3):1071-1079).
      Biological Information
      ImmunogenSepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3b of human Complement C3b/iC3b.
      EpitopeiC3b
      Clone3E7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis clone blocks the activation of alternative pathway of complement (APC) by binding C3b and iC3b.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Primate
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C3
      • CPAMD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight187 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceFlow Cytometry Analysis: This antibody (200 ug mAb/5 x 10E6 cells/mL) detected C3b/iC3b deposit on human Burkett's lymphoma Raji B cells opsonized with anti-CD20 mAb Rituximab (RTX) in the presence of 50% normal human serum (NHS).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Complement C3b/iC3b, -Q2583305 Q2583305

      References

      Reference overviewPub Med ID
      A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement.
      Lindorfer, MA; Pawluczkowycz, AW; Peek, EM; Hickman, K; Taylor, RP; Parker, CJ
      Blood 115 2283-91 2010

      Show Abstract
      20068220 20068220
      An anti-C3b(i) mAb enhances complement activation, C3b(i) deposition, and killing of CD20+ cells by rituximab.
      Kennedy, AD; Solga, MD; Schuman, TA; Chi, AW; Lindorfer, MA; Sutherland, WM; Foley, PL; Taylor, RP
      Blood 101 1071-9 2003

      Show Abstract
      12393727 12393727

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies