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MAB3302 | Anti-Collagen Type V Antibody, clone V-3C9

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Rb ELISA, WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMAB3302
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Collagen Type V Antibody, clone V-3C9
      Product Information
      PresentationLiquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin.
      ApplicationDetect Collagen Type V using this Anti-Collagen Type V Antibody, clone V-3C9 validated for use in ELISA, WB, IH(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoblotting: 5-10μg/mL, reducing conditions, detects 160kDa band.

      Immunohistochemistry on PLP fixed, paraffin-embedded tissues: 1-10 μg/mL. PLP fixation is required; antigen retrieval is HEIR. Not recommended for traditional formalin fixation; acetone fixation can also be used.

      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThe antibody specifically reacts with the hCL(V). This is a purified mouse monoclonal antibody to human type V collagen (hCL(V)).


      This antibody cross-reacts with rabbit and rat CL(V).
      Species Reactivity
      • Human
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
      Gene Symbol
      • COL5A1
      • OTTHUMP00000064637
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20908 # Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
      SIZE: 1838 amino acids; 183560 Da
      SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Sulfated on 40% of tyrosines.
      DISEASE: SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS-I is a connective-tissue disorder characterized by loose-jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. Inheritance is autosomal dominant. & Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type II (EDS-II) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. Inheritance is autosomal dominant.
      SIMILARITY: SwissProt: P20908 ## Belongs to the fibrillar collagen family. & Contains 1 laminin G-like domain. & Contains 1 TSP N-terminal (TSPN) domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number


      Reference overviewPub Med ID
      Exogenous interleukin-6, interleukin-13, and interferon-γ provoke pulmonary abnormality with mild edema in enterovirus 71-infected mice.
      Huang, SW; Lee, YP; Hung, YT; Lin, CH; Chuang, JI; Lei, HY; Su, IJ; Yu, CK
      Respiratory research 12 147 2011

      Show Abstract Full Text Article
      22054060 22054060
      Formaldehyde-inactivated human enterovirus 71 vaccine is compatible for co-immunization with a commercial pentavalent vaccine.
      Chen CW, Lee YP, Wang YF, Yu CK
      Vaccine 29 2772-6. Epub 2011 Feb 11. 2011

      Show Abstract
      21315698 21315698
      Characterization of type I, III and V collagens in high-density cultured tenocytes by triple-immunofluorescence technique.
      Güngörmüş, C; Kolankaya, D
      Cytotechnology 58 145-52 2008

      Show Abstract
      19153816 19153816
      Human placenta type V collagens. Evidence for the existence of an alpha 1(V) alpha 2(V) alpha 3(V) collagen molecule.
      Niyibizi, C, et al.
      J. Biol. Chem., 259: 14170-4 (1984) 1984

      Show Abstract
      6501291 6501291
      Isolation and partial characterization of basement membrane-like collagens from bovine thoracic aorta.
      Mayne, R, et al.
      Artery, 7: 262-80 (1980) 1980

      Show Abstract
      7213017 7213017

      Data Sheet

      Anti-Collagen Type V, clone V-3C9 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies