AB769 Anti-Collagen Type IV Antibody

AB769
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M DB, ELISA, ICC, IHC Gt Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB769
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Collagen Type IV Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • Positive Control: Kidney, muscle, tendon spleen tissue
        Negative Control: Neurons/glia
      PresentationAffinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose™ 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
      Applications
      ApplicationDetect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
      Key Applications
      • Dot Blot
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesDot and slot blotting: 1:100-1:500

      ELISA: 1:1000-1:4000

      Indirect immunohistochemistry (frozen sections only): 1:10-1:40

      Immunocytochemistry 1:10-1:20

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman and bovine placental collagen type IV
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGoat
      SpecificityRecognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
      Gene Symbol
      • COL4A1
      • Arresten
      • arresten
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02462 # Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits endothelial cell proliferation and angiogenesis. Inhibits angiogenesis potentially via mechanisms involving cell surface proteoglycans and the alpha and beta integrins of endothelial cells.
      SIZE: 1669 amino acids; 160615 Da
      SUBUNIT: There are six type IV collagen isoforms, alpha 1(IV)- alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane.
      TISSUE SPECIFICITY: Highly expressed in placenta.
      DOMAIN: SwissProt: P02462 Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G- X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
      PTM: Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates. & Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. & The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues.
      DISEASE: SwissProt: P02462 # Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. & Defects in COL4A1 are a cause of porencephaly type 1 [MIM:175780]; also known as encephaloclastic porencephaly. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Inheritance is autosomal dominant.
      SIMILARITY: Belongs to the type IV collagen family. & Contains 2 COL4C (collagen IV C-terminal) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-TYPE IV COLLAGEN2483202
      GOAT ANTI-TYPE IV COLLAGEN -31660533166053
      GOAT ANTI-TYPE IV COLLAGEN POLYCLONAL ANTIBODY -
      GOAT ANTI-TYPE IV COLLAGEN POLYCLONAL ANTIBODY - 23877392387739
      GOAT ANTI-TYPE IV COLLAGEN POLYCLONAL ANTIBODY - 23970632397063
      GOAT ANTI-TYPE IV COLLAGEN POLYCLONAL ANTIBODY - 24491972449197
      GOAT ANTI-TYPE IV COLLAGEN - 29305442930544
      GOAT ANTI-TYPE IV COLLAGEN - 31670003167000
      GOAT ANTI-TYPE IV COLLAGEN - 31821103182110
      GOAT ANTI-TYPE IV COLLAGEN - 32005803200580

      References

      Reference overviewApplicationSpeciesPub Med ID
      Macromolecular organization and fine structure of the human basilar membrane - RELEVANCE for cochlear implantation.
      Liu, W; Atturo, F; Aldaya, R; Santi, P; Cureoglu, S; Obwegeser, S; Glueckert, R; Pfaller, K; Schrott-Fischer, A; Rask-Andersen, H
      Cell and tissue research  360  245-62  2015

      Show Abstract
      25663274 25663274
      Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing.
      Santos, JM; Camões, SP; Filipe, E; Cipriano, M; Barcia, RN; Filipe, M; Teixeira, M; Simões, S; Gaspar, M; Mosqueira, D; Nascimento, DS; Pinto-do-Ó, P; Cruz, P; Cruz, H; Castro, M; Miranda, JP
      Stem cell research & therapy  6  90  2015

      Show Abstract
      25956381 25956381
      The pre- and post-somatic segments of the human type I spiral ganglion neurons--structural and functional considerations related to cochlear implantation.
      Liu, W; Edin, F; Atturo, F; Rieger, G; Löwenheim, H; Senn, P; Blumer, M; Schrott-Fischer, A; Rask-Andersen, H; Glueckert, R
      Neuroscience  284  470-82  2015

      Show Abstract
      Immunohistochemistry25316409 25316409
      LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis.
      Ray, S; Fanti, JA; Macedo, DP; Larsen, M
      Molecular biology of the cell  25  2393-407  2014

      Show Abstract
      24966172 24966172
      In vitro modeling of the neurovascular environment by coculturing adult human brain endothelial cells with human neural stem cells.
      Chou, CH; Sinden, JD; Couraud, PO; Modo, M
      PloS one  9  e106346  2014

      Show Abstract
      25187991 25187991
      Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
      Öhlund, D; Franklin, O; Lundberg, E; Lundin, C; Sund, M
      BMC cancer  13  154  2013

      Show Abstract
      23530721 23530721
      The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin.
      Driskell, I; Oda, H; Blanco, S; Nascimento, E; Humphreys, P; Frye, M
      The EMBO journal  31  616-29  2012

      Show Abstract
      22117221 22117221
      ROCK1-directed basement membrane positioning coordinates epithelial tissue polarity.
      Daley, WP; Gervais, EM; Centanni, SW; Gulfo, KM; Nelson, DA; Larsen, M
      Development (Cambridge, England)  139  411-22  2012

      Show Abstract
      22186730 22186730
      Localization of AQP5 during development of the mouse submandibular salivary gland.
      Larsen, HS; Aure, MH; Peters, SB; Larsen, M; Messelt, EB; Kanli Galtung, H
      Journal of molecular histology  42  71-81  2011

      Show Abstract
      21203896 21203896
      A focal adhesion protein-based mechanochemical checkpoint regulates cleft progression during branching morphogenesis.
      Daley, WP; Kohn, JM; Larsen, M
      Developmental dynamics : an official publication of the American Association of Anatomists  240  2069-83  2011

      Show Abstract
      22016182 22016182

      Data Sheet

      Title
      GOAT ANTI-TYPE IV COLLAGEN POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies