A part of MilliporeSigma

AB758B | Anti-Collagen Type I Antibody, biotin conjugated

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H DB, ELISA, WB, IHC Gt Biotin Polyclonal Antibody
      Catalogue NumberAB758B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Collagen Type I Antibody, biotin conjugated
      Product Information
      PresentationAffinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.
      ApplicationAnti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
      Key Applications
      • Dot Blot
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesDot and slot blotting: 1:500-1:1,000

      ELISA: 1:1,000-4,000

      Indirect immunohistochemistry (frozen sections only): 1:20-1:40

      The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

      Not recommended for paraffin tissue sections.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman placental collagen type I
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityHuman type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
      Species Reactivity
      • Bovine
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
      Gene Symbol
      • COL1A1
      • OI4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02452 # Type I collagen is a member of group I collagen (fibrillar forming collagen).
      SIZE: 1464 amino acids; 138911 Da
      SUBUNIT: Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
      PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
      DISEASE: SwissProt: P02452 # Defects in COL1A1 are the cause of Caffey disease [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. & Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. Ehlers-Danlos syndrome is a genetically and phenotypically heterogeneous connective-tissue disorder characterized by loose- jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. EDS-I is an autosomal dominant trait. & Defects in COL1A1 are a cause of autosomal dominant Ehlers-Danlos syndrome type VII (EDS-VII) [MIM:130060]; which includes also Ehlers-Danlos syndrome type VII-A1. EDS-VII is characterized by arthrochalasis multiplex congenita, skin hyperextensibility and bruisability. & Defects in COL1A1 are a cause of osteogenesis imperfecta type I (OI-I) [MIM:166200]. OI-I is a dominantly inherited serious newborn disease characterized by bone fragility, normal stature, little or no deformity, blue sclerae and hearing loss in 50% of families. Dentinogenesis imperfecta is rare and may distinguish a subset of OI type I (formation of dentine). & Defects in COL1A1 are a cause of osteogenesis imperfecta type II (OI-II) [MIM:166210]; also known as osteogenesis imperfecta congenita. OI-II is lethal in the perinatal period and is charaterized by calvarial mineralization, beaded ribs, compressed femurs, marked long bone deformity and platyspondyly (congenital flattening of the vertebral bodies). & Defects in COL1A1 are a cause of osteogenesis imperfecta type III (OI-III) [MIM:259420]; also called progressively deforming osteogenesis imperfecta with normal sclerae. OI-III is characterized by progressively deforming bones, usually with moderate deformity at birth, sclerae is variable in color, dentinogenesis imperfecta and hearing loss are common. The stature is very short. & Defects in COL1A1 are a cause of osteogenesis imperfecta type IV (OI-IV) [MIM:166220]. OI-IV is charaterized by normal sclerae, moderate to mild deformity and variable short stature. Dentinogenesis imperfecta is common and hearing loss occurs in some patients. & Genetic variations in COL1A1 are associated with susceptibility to involutional osteoporosis [MIM:166710]; also known as senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture. & A chromosomal aberration involving COL1A1 is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with PDGF. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
      SIMILARITY: SwissProt: P02452 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-COLLAGEN TYPE I -2585895 2585895
      GOAT ANTI-COLLAGEN TYPE I -2762240 2762240
      GOAT ANTI-COLLAGEN TYPE I -2793665 2793665
      GOAT ANTI-COLLAGEN TYPE I B -2812326 2812326


      Reference overviewPub Med ID
      Beneficial Regulation of Fibrillar Collagens, Heat Shock Protein-47, Elastin Fiber Components, Transforming Growth Factor-β1, Vascular Endothelial Growth Factor and Oxidative Stress Effects by Copper in Dermal Fibroblasts.
      Neena Philips,Philips Samuel,Harit Parakandi,Sesha Gopal,Halyna Siomyk,Abraham Ministro,Terrel Thompson,Gadi Borkow
      Connective tissue research 53 2012

      Show Abstract
      22324999 22324999
      Increased expression of TSH receptor by fibrocytes in thyroid-associated ophthalmopathy leads to chemokine production.
      Erin F Gillespie,Konstantinos I Papageorgiou,Roshini Fernando,Nupur Raychaudhuri,Kimberly P Cockerham,Laya K Charara,Allan C P Goncalves,Shuang-Xia Zhao,Anna Ginter,Ying Lu,Terry J Smith,Raymond S Douglas
      The Journal of clinical endocrinology and metabolism 97 2012

      Show Abstract
      22399514 22399514
      Interleukin-6 production in CD40-engaged fibrocytes in thyroid-associated ophthalmopathy: involvement of Akt and NF-κB.
      Gillespie, EF; Raychaudhuri, N; Papageorgiou, KI; Atkins, SJ; Lu, Y; Charara, LK; Mester, T; Smith, TJ; Douglas, RS
      Investigative ophthalmology & visual science 53 7746-53 2012

      Show Abstract
      23092922 23092922

      Data Sheet


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      Alternative Format

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      Life Science Research > Antibodies and Assays > Primary Antibodies