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CP45 | Anti-Clathrin Heavy Chain Mouse mAb (X22)

CP45
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      CP45-100UL
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      Stocked 
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      Available
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the ~180 kDa clathrin heavy chain protein.
          Catalogue NumberCP45
          Brand Family Calbiochem®
          References
          ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
          Zhang, J.Z., et al. 1994. Cell 78, 751.
          Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
          Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
          Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
          Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (1:500)
          Immunofluorescence (1:1000, indirect)
          Immunoprecipitation (see comments)
          Application CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenclathrin heavy chain purified from human brain
          ImmunogenHuman
          CloneX22
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          CP45

          References

          Reference overview
          Bauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
          Zhang, J.Z., et al. 1994. Cell 78, 751.
          Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
          Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
          Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
          Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-August-2007 RFH
          ApplicationImmunoblotting (1:500)
          Immunofluorescence (1:1000, indirect)
          Immunoprecipitation (see comments)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
          BackgroundAt the unstimulated presynaptic nerve terminal, a pool of synaptic vesicles remains docked at active zones in preparation for exocytosis. Nerve terminal depolarization induced through an action potential stimulates the rise in intracellular Ca2+, which ultimately initiates the exocytotic process. Fused synaptic vesicles are rapidly replaced with vesicles from the reserve pool, while membranes of the fused vesicles are recaptured by endocytosis. Clathrin is one component involved in vesicle endocytosis, and is composed of three polypeptides, a ~180 kDa heavy chain and two ~32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology that allows clathrin to form its unique polyhedral network. The clathrin-adaptor AP2 and the AP180/AP3 proteins also play a role in this process by forming a protein layer between the membrane and clathrin lattice. The interaction of the AP2 heterotetramer with synaptotagmin is believed to act as the template for recruitment of the AP3 and clathrin triskelia complex. Progression from clathrin-coated to free vesicles in the nerve terminus also requires dynamin.
          HostMouse
          Immunogen speciesHuman
          Immunogenclathrin heavy chain purified from human brain
          CloneX22
          IsotypeIgG₁
          Speciesbovine, human, mouse, rat
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsImmunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBauerfeind, R., et al. 1996. J. Neurocytol. 25, 701.
          Zhang, J.Z., et al. 1994. Cell 78, 751.
          Herskovits, J.S., et al. 1993. J. Cell Biol. 122, 565.
          Van Der Bliek, A.M., et al. 1993. J. Cell Biol. 122, 553.
          Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol. 6, 151.
          Brodsky, F.M. 1985. J. Cell Biol. 101, 2047.