MAB1254B Anti-Chymase Antibody, clone B7, biotin conjugated

MAB1254B
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H DB, IHC M Biotin Monoclonal Antibody
      Description
      Catalogue NumberMAB1254B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chymase Antibody, clone B7, biotin conjugated
      Alternate Names
      • Mast Cell Protease I
      Background InformationMast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
      References
      Product Information
      FormatBiotin
      Control
      • POSITIVE CONTROL: lung, heart, skin, placenta.
      PresentationCulture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.
      Applications
      ApplicationThis Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
      Key Applications
      • Dot Blot
      • Immunohistochemistry
      Applications Not Recommended
      • Western Blotting
      Application NotesImmunohistochemistry at 1:2,000:1:4,000.

      For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

      Not recommended for use on Western blots.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenChymase purified from human skin.
      CloneB7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with human mast cell chymase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases.
      Gene Symbol
      • CMA1
      • CYM
      • MGC119890
      • Alpha-chymase
      • MCT1
      • MGC119891
      • CYH
      • EC 3.4.21.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23946 # Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
      SIZE: 247 amino acids; 27325 Da
      SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Mast cell granules.
      TISSUE SPECIFICITY: Mast cells in lung, heart, skin and placenta.
      SIMILARITY: SwissProt: P23946 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN MAST CELL CHYMASE -26217322621732
      MOUSE ANTI-HUMAN MAST CELL CHYMASE -26644472664447
      MOUSE ANTI-HUMAN MAST CELL CHYMASE Biotin Conjugated - 25139642513964
      MOUSE ANTI-HUMAN MAST CELL CHYMASE MONOCLONAL ANTIBODY Biotin Conjugated2896064
      MOUSE ANTI-HUMAN MAST CELL CHYMASE MONOCLONAL ANTIBODY Biotin Conjugated2895650
      MOUSE ANTI-HUMAN MAST CELL CHYMASE MONOCLONAL ANTIBODY Biotin Conjugated3142456
      MOUSE ANTI-HUMAN MAST CELL CHYMASE MONOCLONAL ANTIBODY Biotin Conjugated - 22727192272719
      MOUSE ANTI-HUMAN MAST CELL CHYMASE, Monoclonal Antibody, Biotin Conjugated2832216

      References

      Reference overviewPub Med ID
      Phenotypic change and accumulation of smooth muscle cells in strictures in Crohn's disease: relevance to local angiotensin II system.
      Takehisa Suekane,Yoshihiro Ikura,Kenji Watanabe,Junko Arimoto,Yoko Iwasa,Yoshimi Sugama,Soichiro Kayo,Kenichi Sugioka,Takahiko Naruko,Kiyoshi Maeda,Kosei Hirakawa,Tetsuo Arakawa,Makiko Ueda
      Journal of gastroenterology  45  2010

      Show Abstract
      20361214 20361214
      Critical role of mast cell chymase in mouse abdominal aortic aneurysm formation.
      Sun, J; Zhang, J; Lindholt, JS; Sukhova, GK; Liu, J; He, A; Abrink, M; Pejler, G; Stevens, RL; Thompson, RW; Ennis, TL; Gurish, MF; Libby, P; Shi, GP
      Circulation  120  973-82  2009

      Show Abstract
      19720934 19720934
      Mast cells in diffuse large B-cell lymphoma; their role in fibrosis.
      H Fukushima, M Ohsawa, Y Ikura, T Naruko, Y Sugama, T Suekane, C Kitabayashi, T Inoue, M Hino, M Ueda
      Histopathology  49  498-505  2006

      Show Abstract
      17064296 17064296
      Highly increased numbers of leukocytes in inflamed gingiva from patients with HIV infection.
      Maung Myint, Svein Steinsvoll, Zuanning N Yuan, Berit Johne, Kristen Helgeland, Karl Schenck
      AIDS (London, England)  16  235-43  2002

      Show Abstract
      11807308 11807308
      Increased mast cells in hepatocellular carcinoma and intrahepatic cholangiocarcinoma
      Terada, T. and Matsunaga, Y.
      J. Hepatology, 33:961-966 (2000)  2000

      11131459 11131459
      A functional proteomics screen of proteases in colorectal carcinoma.
      McKerrow, JH; Bhargava, V; Hansell, E; Huling, S; Kuwahara, T; Matley, M; Coussens, L; Warren, R
      Molecular medicine (Cambridge, Mass.)  6  450-60  2000

      Show Abstract
      10952024 10952024
      Mast cell subpopulations in the synovial tissue of patients with osteoarthritis: selective increase in numbers of tryptase-positive, chymase-negative mast cells.
      Buckley, M G, et al.
      J. Pathol., 186: 67-74 (1998)  1998

      Show Abstract
      9875142 9875142
      Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptase
      KleinJan, A. et al.
      Allergy, 51:614-620 (1996)  1996

      8899113 8899113
      Mast cells in cutaneous mastocytosis: accumulation of the MCTC type.
      Irani, A A, et al.
      Clin. Exp. Allergy, 20: 53-8 (1990)  1990

      Show Abstract
      2310982 2310982
      Human conjunctival mast cells: distribution of MCT and MCTC in vernal conjunctivitis and giant papillary conjunctivitis
      Irani, A. et al.
      J. Allergy Clin. Immunol., 86:34-39 (1990)  1990

      2196302 2196302

      Data Sheet

      Title
      Anti-Chymase, Clone B7, Biotin Conjugated - Data Sheet

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      Alternative Format

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies