MAB1254 Anti-Chymase Antibody, clone B7

MAB1254
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H DB, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1254
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chymase Antibody, clone B7
      Alternate Names
      • Mast Cell Protease I
      Background InformationMast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: lung, heart, skin, placenta.
      PresentationPurified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
      Applications
      ApplicationAnti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
      Key Applications
      • Dot Blot
      • Immunohistochemistry
      Applications Not Recommended
      • Western Blotting
      Application NotesDot blotting: 0.5-1.0 μg/mL.

      Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

      Not recommended for use on Western blots.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenChymase purified from human skin.
      CloneB7
      Concentration2.4 mg/mL
      HostMouse
      SpecificityReacts with human mast cell chymase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases.
      Gene Symbol
      • CMA1
      • CYM
      • MGC119890
      • Alpha-chymase
      • MCT1
      • MGC119891
      • CYH
      • EC 3.4.21.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23946 # Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
      SIZE: 247 amino acids; 27325 Da
      SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Mast cell granules.
      TISSUE SPECIFICITY: Mast cells in lung, heart, skin and placenta.
      SIMILARITY: SwissProt: P23946 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Chymase, clone B72465148
      Anti-Chymase, clone B7 - 23903352390335
      Anti-Chymase, clone B7 - 24284872428487
      Anti-Chymase, clone B7 - 23021512302151
      Anti-Chymase, clone B7 - 28866872886687
      Anti-Chymase, clone B7 - NG1859617NG1859617
      Anti-Chymase, clone B7 -25931672593167
      Anti-Chymase, clone B7 -25943212594321
      Anti-Chymase, clone B7 -26315382631538
      Anti-Chymase, clone B7 -26694902669490

      References

      Reference overviewApplicationPub Med ID
      Innervation of enteric mast cells by primary spinal afferents in guinea pig and human small intestine.
      Wang, GD; Wang, XY; Liu, S; Qu, M; Xia, Y; Needleman, BJ; Mikami, DJ; Wood, JD
      American journal of physiology. Gastrointestinal and liver physiology  307  G719-31  2014

      Show Abstract
      Immunohistochemistry25147231 25147231
      Involvement of mast cells in gastritis caused by Helicobacter pylori: a potential role in epithelial cell apoptosis.
      Hofman, V; Lassalle, S; Selva, E; Kalem, K; Steff, A; Hébuterne, X; Sicard, D; Auberger, P; Hofman, P
      Journal of clinical pathology  60  600-7  2007

      Show Abstract Full Text Article
      17557865 17557865
      Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.
      Manikandan Jayapal, Hwee Kee Tay, Renji Reghunathan, Liang Zhi, Kah Kiong Chow, Mary Rauff, Alirio J Melendez
      BMC genomics  7  210  2006

      Show Abstract Full Text Article
      16911805 16911805
      Buffy coat preparation is a convenient source of progenitors for culturing mature human mast cells.
      X S Wang, K H Yip, S W Sam, H Y A Lau
      Journal of immunological methods  309  69-74  2006

      Show Abstract
      16413031 16413031
      Co-cultivation of mast cells and Fc epsilon RI alpha+ dendritic-like cells from human hip bone marrow.
      D Kaur, P Berger, S M Duffy, C E Brightling, P Bradding
      Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology  35  226-33  2005

      Show Abstract
      15725196 15725196
      Mast cell chymase expression in Helicobacter pylori-associated gastritis.
      T Matsuo, Y Ikura, M Ohsawa, M Ogami, S Kayo, N Yoshimi, E Hai, T Naruko, M Ohishi, K Higuchi, T Arakawa, M Ueda
      Histopathology  43  538-49  2003

      Show Abstract
      14636254 14636254
      The relative contribution of mast cell subsets to conjunctival TH2-like cytokines.
      D F Anderson, S Zhang, P Bradding, J I McGill, S T Holgate, W R Roche, D F Anderson, S Zhang, P Bradding, J I McGill, S T Holgate, W R Roche
      Investigative ophthalmology visual science  42  995-1001  2001

      Show Abstract
      11274077 11274077
      Increased mast cells in hepatocellular carcinoma and intrahepatic cholangiocarcinoma
      Terada, T. and Matsunaga, Y.
      J. Hepatology, 33:961-966 (2000)  2000

      11131459 11131459
      Expression of angiotensin II and interleukin 6 in human coronary atherosclerotic plaques: potential implications for inflammation and plaque instability.
      B Schieffer, E Schieffer, D Hilfiker-Kleiner, A Hilfiker, P T Kovanen, M Kaartinen, J Nussberger, W Harringer, H Drexler
      Circulation  101  1372-8  2000

      Show Abstract
      10736279 10736279
      Mast cell subpopulations in the synovial tissue of patients with osteoarthritis: selective increase in numbers of tryptase-positive, chymase-negative mast cells.
      Buckley, M G, et al.
      J. Pathol., 186: 67-74 (1998)  1998

      Show Abstract
      9875142 9875142

      Data Sheet

      Title
      Anti-Chymase, clone B7 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies