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MAB5284 | Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301

MAB5284
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, H, Ht, R, Fe, Gr, Pm IP, WB, ICC, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5284
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301
      Alternate Names
      • CSPG
      Background InformationThe antigen recognized by this clone, CAT-301 is a proteoglycan that is developmentaly regulated, high molecular chondroitin sulphate proteoglycan found on the extracellular surface of mammalian neurons. It is expressed late in development and although no definitive role has been identified for CAT-301, it is believed to have a role in the stabilisation of synaptic structure.

      The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly



      Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect



      CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
      Applications
      ApplicationAnti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 detects level of Chondroitin Sulfate Proteoglycan & has been published & validated for use in IP, WB, IC, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:500-1:2,000 on 4% paraformaldehyde fixed frozen tissue.

      Immunocytochemistry

      Immunoblot

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenFeline spinal cord fixed gray matter.
      EpitopeBrain (core protein)
      CloneCat-301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityChondroitin sulfate proteoglycan (CSPG), brain core protein.
      IsotypeIgG1
      Species Reactivity
      • Guinea Pig
      • Human
      • Hamster
      • Rat
      • Feline
      • Gerbil
      • Primate
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN -2701013 2701013
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN MONOCLONAL ANTIBODY - 2388960 2388960
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) -2618337 2618337
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) -2793623 2793623
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN -2512441 2512441
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN MONOCLONAL ANTIBODY - 2025326 2025326
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN MONOCLONAL ANTIBODY - 2070824 2070824

      References | 22 Available | See All References

      Reference overviewApplicationPub Med ID
      Restricted loss of olivocochlear but not vestibular efferent neurons in the senescent gerbil (Meriones unguiculatus).
      Radtke-Schuller, S; Seeler, S; Grothe, B
      Frontiers in aging neuroscience 7 4 2015

      Show Abstract
      25762929 25762929
      Aggrecan and chondroitin-6-sulfate abnormalities in schizophrenia and bipolar disorder: a postmortem study on the amygdala.
      Pantazopoulos, H; Markota, M; Jaquet, F; Ghosh, D; Wallin, A; Santos, A; Caterson, B; Berretta, S
      Translational psychiatry 5 e496 2015

      Show Abstract
      25603412 25603412
      Saccadic Palsy following Cardiac Surgery: Possible Role of Perineuronal Nets.
      Eggers, SD; Horn, AK; Roeber, S; Härtig, W; Nair, G; Reich, DS; Leigh, RJ
      PloS one 10 e0132075 2015

      Show Abstract
      26135580 26135580
      Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment.
      Silver, DJ; Siebzehnrubl, FA; Schildts, MJ; Yachnis, AT; Smith, GM; Smith, AA; Scheffler, B; Reynolds, BA; Silver, J; Steindler, DA
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 15603-17 2013

      Show Abstract
      Immunohistochemistry24068827 24068827
      Molecular compartmentalization of lateral geniculate nucleus in the gray squirrel (Sciurus carolinensis).
      Felch, DL; Van Hooser, SD
      Frontiers in neuroanatomy 6 12 2012

      Show Abstract
      22514523 22514523
      Perisynaptic aggrecan-based extracellular matrix coats in the human lateral geniculate body devoid of perineuronal nets.
      D Lendvai,M Morawski,G Brückner,L Négyessy,G Baksa,T Glasz,L Patonay,R T Matthews,T Arendt,A Alpár
      Journal of neuroscience research 90 2012

      Show Abstract
      21959900 21959900
      Alterations in chondroitin sulfate proteoglycan expression occur both at and far from the site of spinal contusion injury.
      Andrews, EM; Richards, RJ; Yin, FQ; Viapiano, MS; Jakeman, LB
      Experimental neurology 235 174-87 2012

      Show Abstract
      21952042 21952042
      Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner.
      Orlando, C; Ster, J; Gerber, U; Fawcett, JW; Raineteau, O
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 18009-17, 18017a 2012

      Show Abstract
      23238717 23238717
      Atoh1-lineal neurons are required for hearing and for the survival of neurons in the spiral ganglion and brainstem accessory auditory nuclei.
      SM Maricich, A Xia, EL Mathes, VY Wang, JS Oghalai, B Fritzsch, HY Zoghbi
      The Journal of neuroscience : the official journal of the 29 11123-11133 2009

      Show Abstract Full Text Article
      19741118 19741118
      Binge-like postnatal alcohol exposure triggers cortical gliogenesis in adolescent rats.
      Helfer, JL; Calizo, LH; Dong, WK; Goodlett, CR; Greenough, WT; Klintsova, AY
      The Journal of comparative neurology 514 259-71 2009

      Show Abstract Full Text Article
      19296475 19296475

      Data Sheet

      Title
      MOUSE ANTI-CHONDROITIN SULFATE PROTEOGLYCAN (CSPG) BRAIN CORE PROTEIN MONOCLONAL ANTIBODY - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies