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AB144 | Anti-Choline Acetyltransferase Antibody

AB144
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, M, R IHC, WB Gt Serum Polyclonal Antibody
      Description
      Catalogue NumberAB144
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      Alternate Names
      • ChAT
      Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
      References
      Product Information
      FormatSerum
      Control
      • Rat brain tissue
      PresentationGoat antisera, liquid, no preservatives.
      Applications
      ApplicationAnti-Choline Acetyltransferase Antibody is an antibody against Choline Acetyltransferase for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunoblot: 1:1,000-1:5,000

      Immunohistochemistry: 1:500-1:1,000. See page two of this data sheet.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGoat
      SpecificityRecognizes cholinergic neurons in brain and central nervous systems.
      Species Reactivity
      • Guinea Pig
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 2387461 2387461
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) -2601717 2601717
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) -2654600 2654600
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) -2718325 2718325
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) -2762374 2762374
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 1988278 1988278
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 2070812 2070812
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 2201406 2201406
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 2287697 2287697
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 2358833 2358833
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY-2488801 2488801

      References | 48 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Anatomical Location of the Mesencephalic Locomotor Region and Its Possible Role in Locomotion, Posture, Cataplexy, and Parkinsonism.
      Sherman, D; Fuller, PM; Marcus, J; Yu, J; Zhang, P; Chamberlin, NL; Saper, CB; Lu, J
      Frontiers in neurology 6 140 2015

      Show Abstract
      26157418 26157418
      Catecholaminergic innervation of central and peripheral auditory circuitry varies with reproductive state in female midshipman fish, Porichthys notatus.
      Forlano, PM; Ghahramani, ZN; Monestime, CM; Kurochkin, P; Chernenko, A; Milkis, D
      PloS one 10 e0121914 2015

      Show Abstract
      25849450 25849450
      Acute oral administration of low doses of methylphenidate targets calretinin neurons in the rat septal area.
      García-Avilés, Á; Albert-Gascó, H; Arnal-Vicente, I; Elhajj, E; Sanjuan-Arias, J; Sanchez-Perez, AM; Olucha-Bordonau, F
      Frontiers in neuroanatomy 9 33 2015

      Show Abstract
      25852493 25852493
      A genetic and computational approach to structurally classify neuronal types.
      Sümbül, U; Song, S; McCulloch, K; Becker, M; Lin, B; Sanes, JR; Masland, RH; Seung, HS
      Nature communications 5 3512 2014

      Show Abstract
      24662602 24662602
      Neuronal activity (c-Fos) delineating interactions of the cerebral cortex and basal ganglia.
      Qiu, MH; Chen, MC; Huang, ZL; Lu, J
      Frontiers in neuroanatomy 8 13 2014

      Show Abstract
      24723855 24723855
      Expression of mu opioid receptor in dorsal diencephalic conduction system: new insights for the medial habenula.
      Gardon, O; Faget, L; Chu Sin Chung, P; Matifas, A; Massotte, D; Kieffer, BL
      Neuroscience 277 595-609 2014

      Show Abstract
      25086313 25086313
      ZPK/DLK and MKK4 form the critical gateway to axotomy-induced motoneuron death in neonates.
      Itoh, T; Horiuchi, M; Ikeda, RH; Xu, J; Bannerman, P; Pleasure, D; Penninger, JM; Tournier, C; Itoh, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 10729-42 2014

      Show Abstract
      25100604 25100604
      Ldb1 is essential for development of Nkx2.1 lineage derived GABAergic and cholinergic neurons in the telencephalon.
      Zhao, Y; Flandin, P; Vogt, D; Blood, A; Hermesz, E; Westphal, H; Rubenstein, JL
      Developmental biology 385 94-106 2014

      Show Abstract
      24157949 24157949
      Perineuronal and perisynaptic extracellular matrix in the human spinal cord.
      Jäger, C, et al.
      Neuroscience, 238: 168-84 (2013) 2013

      Show Abstract
      23428622 23428622
      Role of inhibition in respiratory pattern generation.
      Janczewski, WA; Tashima, A; Hsu, P; Cui, Y; Feldman, JL
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 5454-65 2013

      Show Abstract
      Immunohistochemistry23536061 23536061

      Data Sheet

      Title
      GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies