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MAB8879 | Anti-Cdk4 Antibody, clone DCS-35

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Po, R IF, IP, EA, WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMAB8879
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cdk4 Antibody, clone DCS-35
      Background InformationThe prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.
      Product Information
      PresentationPurified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.
      ApplicationDetect Cdk4 with Anti-Cdk4 Antibody, clone DCS-35 (Mouse Monoclonal Antibody), that has been shown to work in IF, IP, EA, WB & IHC.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Enzyme Assay
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunofluorescence

      Immunoprecipitation: excellent for coprecipitation of D-type cyclins bound to cdk4.

      Western Blotting: 2-4 ug/mL

      Kinase Assay

      The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRaised against purified recombinant cdk4 protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes a 34 kDa protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks.
      Species Reactivity
      • Human
      • Mouse
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
      Gene Symbol
      • CDK4
      • PSK-J3
      • CMM3
      • MGC14458
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11802 # Probably involved in the control of the cell cycle.
      SIZE: 303 amino acids; 33730 Da
      SUBUNIT: Forms a stable complex with D-type G1 cyclins. Interacts with SEI1 and ZNF655/VIK.
      DISEASE: SwissProt: P11802 # CDK4 mutations are involved in tumor formation. & Defects in CDK4 are the cause of cutaneous malignant melanoma 3 (CMM3) [MIM:609048, 155600]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites.
      SIMILARITY: SwissProt: P11802 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain liquid material at 2-8°C for up to 24 months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-cdk4 MONOCLONAL ANTIBODY - 2115542 2115542
      MOUSE ANTI-cdk4 MONOCLONAL ANTIBODY - 2370564 2370564
      MOUSE ANTI-cdk4 MONOCLONAL ANTIBODY - 2411819 2411819
      MOUSE ANTI-cdk4 -2557559 2557559
      MOUSE ANTI-cdk4 -2695265 2695265
      MOUSE ANTI-cdk4 -2726161 2726161
      MOUSE ANTI-cdk4 -2761671 2761671
      MOUSE ANTI-cdk4 MONOCLONAL ANTIBODY - 2024860 2024860
      MOUSE ANTI-cdk4 MONOCLONAL ANTIBODY - 2208751 2208751


      Reference overviewPub Med ID
      Effects of quercetin on CDK4 mRNA and protein expression in A549 cells infected by H1N1.
      Wan, Q; Wang, H; Lin, Y; Gu, L; Han, M; Yang, Z; Zhang, Y; Ma, R; Wang, L; Wang, Z
      Biomedical reports 1 766-770 2013

      Show Abstract
      24649026 24649026
      Cell cycle regulators cyclin D1 and CDK4/6 have estrogen receptor-dependent divergent functions in breast cancer migration and stem cell-like activity.
      Lamb, R; Lehn, S; Rogerson, L; Clarke, RB; Landberg, G
      Cell cycle (Georgetown, Tex.) 12 2384-94 2013

      Show Abstract
      23839043 23839043
      Angiotensin II regulates activation of Bim via Rb/E2F1 during apoptosis: involvement of interaction between AMPKβ1/2 and Cdk4.
      Kim, YC; Day, RM
      American journal of physiology. Lung cellular and molecular physiology 303 L228-38 2012

      Show Abstract
      22659879 22659879
      Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats.
      Li-Li Tao,Yan Lei,Guo-Li Wang,Ling-Qun Zhu,Yang Wang
      Chinese journal of integrative medicine 18 2012

      Show Abstract
      22855034 22855034
      Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells.
      Xu, B; Zhang, K; Huang, Y
      RNA (New York, N.Y.) 15 357-61 2009

      Show Abstract Full Text Article
      19147696 19147696
      The immunohistochemical profile of the primary and metastatic carcinoma of the clitoris: a case report.
      Andrzej Semczuk, Danuta Skomra, Katarzyna Jankiewicz, Aneta Adamiak, Elzbieta Korobowicz, Tomasz Rechberger
      Archives of gynecology and obstetrics 273 187-91 2005

      Show Abstract
      16167159 16167159
      Expression of the cell-cycle regulatory proteins (pRb, cyclin D1, p16INK4A and cdk4) in human endometrial cancer: correlation with clinicopathological features.
      Andrzej Semczuk, Roman Miturski, Danuta Skomra, Jerzy A Jakowicki
      Archives of gynecology and obstetrics 269 104-10 2004

      Show Abstract
      14648178 14648178

      Data Sheet

      Anti-Cdk4, clone DCS-35 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies