219350 Anti-β-Catenin, Dephospho Mouse mAb (8E4)

219350
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      219350-1SET
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
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          Will advise
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          Description
          OverviewRecognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
          Catalogue Number219350
          Brand Family Calbiochem®
          Application Data
          Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
          Positive controlLiCl-treated SW480 cells (included)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
          Application CommentsSW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
          ImmunogenMouse
          Clone8E4
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          219350

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
          Application Data
          Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
          DescriptionMouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
          Backgroundβ-Catenin is a cytoplasmic protein that mediates the interaction of Ca2+-dependent transmembrane adhesion proteins (cadherins) with the cytoskeletal network. β-Catenin may also play a role in tumorigenesis, as it complexes with the tumor suppressor gene product APC. β-Catenin directly interacts and constitutively activates transcription factors of the TCF/LEF family.
          HostMouse
          Immunogen speciesMouse
          Immunogena synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
          Clone8E4
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlLiCl-treated SW480 cells (included)
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
          CommentsSW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling