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MAB10753 | Anti-Caspase3 (active form) Antibody, clone 3D9.3

MAB10753
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB10753
      Replaces04-1090; 04-439
      DescriptionAnti-Caspase3 (active form) Antibody, clone 3D9.3
      Alternate Names
      • Caspase-3
      • CASP-3
      • Apopain
      • Cysteine protease CPP32
      • CPP-32
      • Protein Yama
      • SREBP cleavage activity 1
      • SCA-1
      • Caspase-3 subunit p17
      • Caspase-3 subunit p12
      Background InformationCaspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
      Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Caspase3 (active form).
      Clone3D9.3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CASP3
      • CPP32
      Purification MethodPurified by Ion-Exchange Chromatography
      UniProt Number
      Molecular Weight~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase3 (active form), clone -2550999 2550999
      Anti-Caspase3 (active form), clone -2807042 2807042
      Anti-Caspase3 (active form), clone 3D9.3 - 2398965 2398965
      Anti-Caspase3 (active form), clone 3D9.3 - Q2116523 Q2116523
      Anti-Caspase3 (active form), clone 3D9.3 -2607646 2607646
      Anti-Caspase3 (active form), clone 3D9.3 -2632554 2632554
      Anti-Caspase3 (active form), clone 3D9.3 -2659366 2659366
      Anti-Caspase3 (active form), clone 3D9.3 -2672468 2672468
      Anti-Caspase3 (active form), clone 3D9.3 -2686144 2686144
      Anti-Caspase3 (active form), clone 3D9.3 -2718260 2718260
      Anti-Caspase3 (active form), clone 3D9.3_2840431 2840431

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies