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AB3629 | Anti-Caspase 9 Antibody, active (cleaved) form

AB3629
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB, ICC Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3629
      Replaces04-443
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 9 Antibody, active (cleaved) form
      Background InformationCaspase-9 is an important member of the caspase family. When cytochrome c is released from the mitochondria early upon apoptosis induction, it associates with Apaf-1 and procaspase-9 and forms an apoptosome. This complex cleaves procaspase-9 into a large 37kDa subunit and a small 10kDa subunit.
      References
      Product Information
      FormatPurified
      PresentationAffinity purified immunoglobulin. Liquid in PBS, containing 50% glycerol, 0.5% BSA and 0.02% thimerosal.
      Applications
      ApplicationAnti-Caspase 9 Antibody, active (cleaved) form detects level of Caspase 9 & has been published & validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot: 0.5-4 μg/mL

      Immunoprecipitation: 10-20 μg/mL

      Immunocytochemistry: 10-20 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide. FQEGLRTFDQLD
      Epitopeactive (cleaved) form
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB3629 detects only the cleaved large 37kDa subunit and does not detect the precursor form in cells undergoing apoptosis. It also does not detect other caspases.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
      Gene Symbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • APAF3
      • MCH6
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC 3.4.22.62 [Contains: Caspase-9 subunit p35
      • Caspase-9 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
      SIZE: 416 amino acids; 46281 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
      PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C. For optimal storage, aliquot and freeze at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-ACTIVE CASPASE-9 -2569710 2569710
      RABBIT ANTI-ACTIVE CASPASE-9 -2703731 2703731
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY - 2466926 2466926
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY - 2006343 2006343
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY - 2188444 2188444
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY - 2204876 2204876
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY - 2303940 2303940
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY -2605598 2605598
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY -2637125 2637125
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY -2695227 2695227
      RABBIT ANTI-ACTIVE CASPASE-9 POLYCLONAL ANTIBODY -2769572 2769572

      References

      Reference overviewPub Med ID
      Geldanamycin induces mitotic catastrophe and subsequent apoptosis in human glioma cells.
      Motohiro Nomura, Naoko Nomura, Elizabeth W Newcomb, Yevgeniy Lukyanov, Cristina Tamasdan, David Zagzag
      Journal of cellular physiology 201 374-84 2004

      Show Abstract
      15389545 15389545

      Data Sheet

      Title
      Anti-Caspase 9, active (cleaved) form - Data Sheet

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      Product Families

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies