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MAB10754 | Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1

MAB10754
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB10754
      Replaces04-573; 04-574
      DescriptionAnti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1
      Alternate Names
      • caspase 8, apoptosis-related cysteine peptidase
      • caspase-8
      • caspase 8, apoptosis-related cysteine protease
      • MACH-beta-1/2/3/4 protein
      • Apoptotic protease Mch-5
      • Apoptotic cysteine protease
      • MACH-alpha-1/2/3 protein
      • FADD-homologous ICE/ced-3-like protease
      • MORT1-associated ced-3 homolog
      • ICE-like apoptotic protease 5
      • FADD-like ICE
      Background InformationCaspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18. Active caspase-8 or the p18 subunit, is the first in the caspase signaling cascade, and it signals downstream caspases to eventually induce apoptosis.
      References
      Product Information
      FormatPurified
      Control
      • Camptothecin-treated and untreated Jurkat lysates
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationDetect Caspase-8 (active form p18 subunit) using this Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected active Caspase-8 (p18 subunit) in etoposide-treated HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the active form of human Caspase-8.
      Clone2B12.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the active form (p18 subunit) of Caspase-8.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq].
      Gene Symbol
      • CASP8
      • MCH5
      • CASP-8
      • MACH
      • ALPS2B
      • FLICE
      • procaspase-8
      • CAP4
      Purification MethodIon Exchange
      UniProt Number
      UniProt SummaryFUNCTION: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

      SIZE: 479 amino acids; 55391 Da

      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.

      PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.

      DISEASE: Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.

      SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
      Molecular Weight~18 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in camptothecin-treated and untreated Jurkat lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected the active form of Caspase-8 (p18 subunit) in 10 µg of camptothecin-treated Jurkat lysates. Untreated jurkat lysate exhibited no detection of active Caspase-8.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - 2562377 2562377
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - 2015586 2015586
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - 2266760 2266760
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - 2327013 2327013
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - NG1951092 NG1951092
      Anti-Caspase-8 (active form p18 subunit), clone 2B12.1 - NRG1854601 NRG1854601

      References

      Reference overviewPub Med ID
      An increase in MECP2 dosage impairs neural tube formation.
      Petazzi, P; Akizu, N; García, A; Estarás, C; Martínez de Paz, A; Rodríguez-Paredes, M; Martínez-Balbás, MA; Huertas, D; Esteller, M
      Neurobiology of disease 67 49-56 2014

      Show Abstract
      24657916 24657916

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies