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MABF265 | Anti-Caspase-4, clone 17D9 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP, IHC R Purified Monoclonal Antibody
      Catalogue NumberMABF265
      DescriptionAnti-Caspase-4, clone 17D9 Antibody
      Alternate Names
      • Caspase-4
      • CASP-4
      • Caspase-11
      • CASP-11
      • Protease ICH-3
      • .Caspase-4 subunit p10
      • Caspase-4 subunit p20
      Background InformationCaspase-4 (CASP-4), also known as Caspase-11 (CASP-11), or Protease ICH-3, and encoded by the gene names Casp4, Casp11, Caspl, and Ich3, is a member of the peptidase C14A family and might be involved in ER-stress induced apoptosis. Caspase-4 protein has been shown to cleave Caspase-1 and might be involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-4 promotes IL-1 beta processing by ICE, and therefore may also play a role in inflammatory responses. Caspase-4 has been indicated to interact with TMEM214, in the process of ER membrane localization. Additionally, Caspase-4 is a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The two subunits are derived by an autocatalytic mechanism, from the precursor sequence. Recent studies have shown that the activity of Caspase-4 is increased 30-fold by endotoxins (LPS). Other studies have indicated that Caspase-4 is induced by ER stress in a DDIT3/CHOP-dependent manner. Caspase-4 is expressed mostly in lung and spleen, and less in liver, skeletal muscle, kidney and testis.
      Product Information
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Caspase-4, clone 17D9 is an antibody against Caspase-4 for use in WB, IP, IH.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Caspase-4 in ischemia induced mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).
      Immunohistochemistry Analysis: A representative lot detected Caspase-4 in wildtype and ischemic mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).
      Biological Information
      ImmunogenCorresponding to mouse Caspase-4.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Casp4
      • Casp11
      • Caspl
      • Ich3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~48 and 38 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in bacterial J53 treated macrophage cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Caspase-4 in 10 µg of bacterial J53 treated macrophage cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase-4, clone 17D9 Q2479834
      Anti-Caspase-4, clone 17D9 -2761307 2761307


      Reference overviewPub Med ID
      Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.
      Kang, SJ; Wang, S; Hara, H; Peterson, EP; Namura, S; Amin-Hanjani, S; Huang, Z; Srinivasan, A; Tomaselli, KJ; Thornberry, NA; Moskowitz, MA; Yuan, J
      The Journal of cell biology 149 613-22 2000

      Show Abstract
      10791975 10791975