A part of MilliporeSigma

AB3612 | Anti-Caspase 12 Antibody, prodomain, aa 100-116

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB3612
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 12 Antibody, prodomain, aa 100-116
      Background InformationThree distinct signaling pathways lead to apoptosis. The death receptor and mitochondrion are the two main pathways which involve caspase-8 and caspase-9, respectively. The endoplasmic reticulum stress is the third apoptotic pathway and involves caspase-12. Localized to the ER but not to cytoplasm or mitochondria, caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including γ-secretase presenilin and ß-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-ß. Caspase-12 is ubiquitously expressed in mouse tissues.
      Product Information
      FormatAffinity Purified
      • Positive: mouse brain tissue lysate
      PresentationImmunoaffinity chromatography purified antibody supplied in PBS containing 0.02% sodium azide.
      ApplicationDetect Caspase 12 using this Anti-Caspase 12 Antibody, prodomain, aa 100-116 validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 0.5 μg - 1 μg. Detects a band at ~49-53 kDa. in normal tissue extracts. Caspase-12 is a stress induced caspase localized to the ER and activated upon ER stress. Active process forms are difficult to detect in most non-transfected systems. Not all cells produce caspase-12; mouse L929 fibrosacrcoma cells are an excellent positive control line.

      Immunohistochemistry: 2 μg /mL in mouse liver tissue

      Mouse brain tissue lysate can be used as a positive control. Caspase 12 is associated with ER membranes and not in cytoplasm or mitochondria. Caspase 12 mediates cytotoxixity induced by amyloid beta.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 100-116 of murine caspase-12.
      Epitopeprodomain, aa 100-116
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes caspase-12 pro-protein in mouse extracts of normal and apoptotic cells. Caspase-12 is predicted to have a large, >120 aa, long prodomain region. Thus this antibody recognizes only the proforms of the enzyme and not the active subunits. Uncleaved (proform) caspase-12 is prominently detected in many tissues. Active forms are difficult to detect expect in transfect cell systems. {Kalai, M et al (2003) 162:457-467}.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryCaspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis.
      Gene Symbol
      • CASP12
      • Caspase-12.
      • CASP12P1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummarySIZE: 341 amino acids; 38907 Da
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2°-8°C for up to one year from date of receipt
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CASPASE-12 -2665000 2665000


      Reference overviewApplicationPub Med ID
      Caspase-12 mediates carbon tetrachloride-induced hepatocyte apoptosis in mice.
      Liu, H; Wang, Z; Nowicki, MJ
      World journal of gastroenterology 20 18189-98 2014

      Show Abstract
      Western Blotting25561786 25561786
      Activation of endoplasmic reticulum stress in degenerating photoreceptors of the rd1 mouse.
      Yang, LP; Wu, LM; Guo, XJ; Tso, MO
      Investigative ophthalmology & visual science 48 5191-8 2007

      Show Abstract
      17962473 17962473
      Endoplasmic reticulum stress-associated caspase 12 mediates cisplatin-induced LLC-PK1 cell apoptosis.
      Liu, H; Baliga, R
      Journal of the American Society of Nephrology : JASN 16 1985-92 2005

      Show Abstract
      15901768 15901768
      Global changes in gene regulation demonstrate that unconjugated bilirubin is able to upregulate and activate select components of the endoplasmic reticulum stress response pathway.
      Garth H Oakes,John R Bend
      Journal of biochemical and molecular toxicology 24 2001

      Show Abstract
      20196124 20196124
      Caspases find a new place to hide.
      Mehmet, H
      Nature, 403: 29-30 (2000) 2000

      10638735 10638735
      Characterization of seven murine caspase family members.
      Van de Craen, M, et al.
      FEBS Lett., 403: 61-9 (1997) 1997

      Show Abstract
      9038361 9038361
      Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors.
      Nakagawa, T, et al.
      J. Neurosurg., 81: 69-77 (1994) 1994

      Show Abstract
      8207529 8207529

      Data Sheet

      Anti-Caspase 12, prodomain, aa 100-116 - Data Sheet

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