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MABS1215 | Anti-Calpain I/II, large subunit Antibody, clone 1D10A7

50 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, Rb, Ch, Mk, H WB, ICC M Ascites Monoclonal Antibody
      Catalogue NumberMABS1215
      DescriptionAnti-Calpain I/II, large subunit Antibody, clone 1D10A7
      Alternate Names
      • Calpain-1 catalytic subunit
      • Calcium-activated neutral proteinase 1
      • CANP 1
      • Calpain mu-type
      • Calpain-1 large subunit
      • Cell proliferation-inducing gene 30 protein
      • Micromolar-calpain
      • muCANP
      • Calpain I/II large subunit
      Background InformationCalpains I & II are intracellular cysteine proteinases distributed in various tissues and cells. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca+2 concentrations and calpain II at millimolar Ca+2 concentrations. Both calpains are composed of a large catalytic (80 kDa) and a small regulatory (30 kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain activity was initially characterized as “calcium-activated neutral protease” (CANP) detected in brain, lens of the eye, and other tissues. The name “calpain” was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.
      Product Information
      PresentationMouse monoclonal IgG2aκ ascites with 0.05% sodium azide.
      ApplicationDetect CAPN1 using this mouse monoclonal Anti-Calpain I/II, large subunit Antibody, clone 1D10A7, Cat. No. MABS1215, validated for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:20,000 dilution from a representative lot detected recombinant human calpain-2 and calpain-1 C115S mutant protein.
      Western Blotting Analysis: A 1:20,000 dilution from a representative lot detected Calpain, large subunit in rat spleen tissue lysate.
      Immunocytochemistry Analysis: A representative lot detected Calpain, large subunit in mouse embryonic fibroblast (MEF) cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207).
      Western Blotting Analysis: A representative lot detected Calpain, large subunit in cytosolic preparation from rat cortical tissue (Blomgren, K., et al. (1995). Brain Res. 684(2):143-149).
      Western Blotting Analysis: A representative lot detected purfied mu- and m-calpain large subunits from rabbit, monkey, human, and rat tissues (Kawashima, S., et al. (1998). Biol Chem. 379(2):201-204).
      Western Blotting Analysis: A representative lot detected Calpain, large subunit in normal & cataractous rat lens homogenates (Inomata, M., et al. (2009). Nat Med. 15(10):1202-1207).
      Western Blotting Analysis: A representative lot detected Calpain, large subunit in MEF cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207).
      Biological Information
      ImmunogenPurified m-calpain from rabbit skeletal muscle.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 1D10A7 detects the large catalytic (80K), but not the small regulatory (30K), subunit of mu-calpain (calpain I) and m-calpain (calpain II), with ~7-fold higher affinity toward m-calpain than mu-calpain.
      Species Reactivity
      • Rat
      • Rabbit
      • Chicken
      • Monkey
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CAPN1
      • CANPL1
      • PIG30
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~80 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat lung tissue lysate.

      Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Calpain, large subunit in rat lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Calpain I/II, large subunit, -Q2583316 Q2583316


      Reference overviewPub Med ID
      Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly.
      Yamada, M; Yoshida, Y; Mori, D; Takitoh, T; Kengaku, M; Umeshima, H; Takao, K; Miyakawa, T; Sato, M; Sorimachi, H; Wynshaw-Boris, A; Hirotsune, S
      Nature medicine 15 1202-7 2009

      Show Abstract
      19734909 19734909
      Isolation and characterization of monoclonal antibodies against calcium-activated neutral protease with low calcium sensitivity.
      Kasai, Y; Inomata, M; Hayashi, M; Imahori, K; Kawashima, S
      Journal of biochemistry 100 183-90 1986

      Show Abstract
      3020013 3020013

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

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