208730 | Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)

208730
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208730-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain.
          Catalogue Number208730
          Brand Family Calbiochem®
          Application Data
          This antibody can be used to immunoprecipitate the small subunit of Calpain I and Calpain II under native and denatured conditions. Immunoprecipitation (10% SDS-PAGE) Samples: A431 cells lysed under native conditions and immunoprecipitated with clone 28F3 anti-calpain I,II small subunit (Cat. No. 208730) (lane 1), an irrelevant antibody (lane 2) and direct lysate (lane 3). Primary antibody concentration: 0.5 µg/ml (Cat. No: 208730) Secondary antibody concentration: 0.2 µg/ml goat anti-mouse IgG-HRP, γ-chain specific (Cat. No. 401207) Exposure: 1 min Obtained results: Bands at ~30 kDa in lanes 1 and 3
          References
          Product Information
          FormLiquid
          FormulationIn 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
          Positive controlMDBK cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (1:2000)
          Immunoblotting (1:2000)
          Immunoprecipitation (1:500)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenpurified calpain from human placenta
          ImmunogenHuman Placenta
          Clone28F3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Bovine
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208730

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Calcium Metabolism and Related Products Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-July-2007 RFH
          ApplicationELISA (1:2000)
          Immunoblotting (1:2000)
          Immunoprecipitation (1:500)
          Application Data
          This antibody can be used to immunoprecipitate the small subunit of Calpain I and Calpain II under native and denatured conditions. Immunoprecipitation (10% SDS-PAGE) Samples: A431 cells lysed under native conditions and immunoprecipitated with clone 28F3 anti-calpain I,II small subunit (Cat. No. 208730) (lane 1), an irrelevant antibody (lane 2) and direct lysate (lane 3). Primary antibody concentration: 0.5 µg/ml (Cat. No: 208730) Secondary antibody concentration: 0.2 µg/ml goat anti-mouse IgG-HRP, γ-chain specific (Cat. No. 401207) Exposure: 1 min Obtained results: Bands at ~30 kDa in lanes 1 and 3
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2).
          BackgroundCalpains are calcium-dependent thiol-proteases that proteolyze a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain-1 (µ-form) and calpain-2 (m-form), differ in their calcium requirement for activation. They are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme, whereas the 30 kDa unit is the regulatory subunit and is common to both µ- and m-isozymes.
          HostMouse
          Immunogen speciesHuman Placenta
          Immunogenpurified calpain from human placenta
          Clone28F3
          IsotypeIgG₁
          Speciesbovine, human, not mouse, not rat
          Positive controlMDBK cells
          FormLiquid
          FormulationIn 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling