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04-1079 | Anti-Calcium/Calmodulin Kinase II Antibody, clone EP1829Y, rabbit monoclonal

04-1079
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC, IH(P), IP, FC Rb Unpurified Monoclonal Antibody
      Description
      Catalogue Number04-1079
      DescriptionAnti-Calcium/Calmodulin Kinase II Antibody, clone EP1829Y, rabbit monoclonal
      Alternate Names
      • CAMKK beta protein
      • CaM-KK beta
      • CaM-kinase kinase beta
      • CaMKK beta
      • Calcium/calmodulin-dependent protein kinase kinase beta
      • calcium/calmodulin-dependent protein kinase beta
      • calcium/calmodulin-dependent protein kinase kinase 2 beta
      • calcium/calmodulin-dependent protein kinase kinase 2, beta
      Background InformationThe Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
      References
      Product Information
      FormatUnpurified
      Control
      • Mouse brain lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationThis Anti-Calcium/Calmodulin Kinase II Antibody, clone EP1829Y, Rabbit is validated for use in WB, IH, IH(P), IP, FC for the detection of Calcium/Calmodulin Kinase II.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Calcium/Calmodulin Kinase II in human brain carcinoma tissue.
      Immunocytochemistry Analysis: A 1:250-500 dilution from a previous lot was used in IC.
      Flow Cytometry: A 1:80 dilution of a previous lot was used in flow cytometry.
      Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
      Immunohistochemistry (Paraffin) Analysis:
      Representative lot data.
      Immunohistochemical staining of paraffin-embedded human brain carcinoma using Anti-Calcium/Calmodulin Kinase II, clone EP1829Y.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on human Calcium/Calmodulin Kinase II.
      CloneEP1829Y
      HostRabbit
      SpecificityThis antibody recognizes Calcium/Calmodulin Kinase II.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CAMKK
      • CAMKKB
      • EC 2.7.11.17
      • KIAA0787
      • MGC15254
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1 By similarity.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca2+/calmodulin. In part, activity is independent on Ca2+/calmodulin By similarity.
      SUBUNIT STRUCTURE: Interacts with calmodulin By similarity.
      SUBCELLULAR LOCATION:Cytoplasm By similarity.
      TISSUE SPECIFICITY: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
      DOMAIN: The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates By similarity.
      PTM: Autophosphorylated By similarity.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

      Molecular Weight~45-70 kDa (2 isoforms)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse brain lysates.
      Western Blot Analysis: A 1:20,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase II in mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2384651 2384651
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2390754 2390754
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2390980 2390980
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2392202 2392202
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - NG1740997 NG1740997
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2066324 2066324
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - 2219384 2219384
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - NG1818072 NG1818072
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - NG1917777 NG1917777
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal - NRG1649492 NRG1649492
      Anti-Calcium/Calmodulin Kinase II, clone EP1829Y, Rabbit Monoclonal -2515377 2515377

      References

      Reference overviewApplicationPub Med ID
      Direct visualization of newly synthesized target proteins in situ.
      tom Dieck, S; Kochen, L; Hanus, C; Heumüller, M; Bartnik, I; Nassim-Assir, B; Merk, K; Mosler, T; Garg, S; Bunse, S; Tirrell, DA; Schuman, EM
      Nature methods 12 411-4 2015

      Show Abstract
      25775042 25775042
      Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells.
      Lucki, NC; Li, D; Sewer, MB
      Molecular and cellular endocrinology 348 165-75 2012

      Show Abstract
      Western Blotting21864647 21864647