681505 Anti-CaV2.2 (851-867) Rabbit pAb

681505
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      681505-1ST
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          Plastic ampoule 1 st
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          Description
          OverviewRecognizes the ~210 kDa and the ~240 kDa forms of α1B-subunit of Cav2.2. Supplied with a control peptide.
          Note: The α-subunit is highly sensitive to proteases. Please refer to the product insert for proper sample preparation.
          Catalogue Number681505
          Brand Family Calbiochem®
          SynonymsAnti-N-Type-Ca2+ Channel
          Application Data
          Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

          Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
          References
          ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
          Product Information
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Application ReferencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
          Key Applications Immunoprecipitation
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunohistochemistry
          Application NotesImmunoblotting (1.5-3 µg/ml)
          Immunocytochemistry (see application references)
          Immunohistochemistry (1:1000; see application references)
          Immunoprecipitation (see application references)
          Application CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-CaV2.2 (851-867) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-CaV2.2 (851-867) Rabbit pAb Certificates of Analysis

          TitleLot Number
          681505

          References

          Reference overview
          Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-November-2009 RFH
          SynonymsAnti-N-Type-Ca2+ Channel
          ApplicationImmunoblotting (1.5-3 µg/ml)
          Immunocytochemistry (see application references)
          Immunohistochemistry (1:1000; see application references)
          Immunoprecipitation (see application references)
          Application Data
          Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

          Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~210 kDa and the ~240 kDa forms the voltage-gated Ca2+ channel of α1B-subunit. Supplied with a control peptide (immunogen).
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
          IsotypeIgG
          Speciesmouse, rat
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
          Application referencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.