681507 Anti-CaV1.2 (818-835) Rabbit pAb

681507
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, Rb, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      681507-1ST
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          Description
          OverviewRecognizes the ~190 kDa and ~210 forms of the α1C-subunit. Supplied with a control peptide. Note: The α-subunit is highly sensitive to proteases. Please refer to the data sheet for proper sample preparation.
          Catalogue Number681507
          Brand Family Calbiochem®
          SynonymsAnti-L-Type Ca2+ Channel
          Application Data
          Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

          Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
          References
          ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
          Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
          Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
          Snutch, T.P. et al. 1991. Neuron 7, 45.
          Product Information
          FormLyophilized
          Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
          Positive controlRat brain or heart
          PreservativeNone
          Applications
          Application ReferencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
          Key Applications Immunoprecipitation
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunohistochemistry
          Application NotesImmunoblotting (1:200)
          Immunocytochemistry (see application references)
          Immunoprecipitation (see application references)
          Immunohistochemistry (!:100)
          Application CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          • Rabbit
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-CaV1.2 (818-835) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-CaV1.2 (818-835) Rabbit pAb Certificates of Analysis

          TitleLot Number
          681507

          References

          Reference overview
          Hell, J.W., et al. 1995. EMBO J. 14, 3036.
          Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
          Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
          Snutch, T.P. et al. 1991. Neuron 7, 45.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-August-2011 JSW
          SynonymsAnti-L-Type Ca2+ Channel
          ApplicationImmunoblotting (1:200)
          Immunocytochemistry (see application references)
          Immunoprecipitation (see application references)
          Immunohistochemistry (!:100)
          Application Data
          Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

          Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
          DescriptionImmunoaffinity purified Rabbit polyclonal antibody. Recognizes the ~190 kDa and the ~210 kDa forms of the α1C-subunit of the voltage-gated Ca2+ channel. Supplied with a control peptide (immunogen).
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
          IsotypeIgG
          Speciesmouse, rabbit, rat
          Positive controlRat brain or heart
          FormLyophilized
          Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
          PreservativeNone
          CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
          Toxicity Standard Handling
          ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
          Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
          Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
          Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
          Snutch, T.P. et al. 1991. Neuron 7, 45.
          Application referencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.