A part of MilliporeSigma

ABN182 | Anti-CYP27B1 Antibody

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABN182
      DescriptionAnti-CYP27B1 Antibody
      Alternate Names
      • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
      • 25-OHD-1 alpha-hydroxylase
      • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
      • VD3 1A hydroxylase
      • Calcidiol 1-monooxygenase
      • Cytochrome P450 subfamily XXVIIB polypeptide 1
      • Cytochrome P450C1 alpha
      • Cytochrome P450VD1-alpha
      • Cytochrome p450 27B1
      Background Information25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth.
      Product Information
      FormatAffinity Purified
      • Serum free MCF-7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human CYP27B1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYP27B1
      • CYP1ALPHA
      • CYP27B
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in serum free MCF-7 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AP182P Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies