AP1135 Anti-CTNNBL1 Mouse mAb (5F1)

AP1135
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1135-50UG
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          Glass bottle 50 μg
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          Description
          OverviewRecognizes the ~65-70 kDa CTNNBL1 protein in HeLa cells and human testes tissue.
          Catalogue NumberAP1135
          Brand Family Calbiochem®
          SynonymsAnti-Beta catenin like protein 1
          Application Data
          Detection of human CTNNBL1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CTNNBL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (40 µg/ml). Detection: fluorescence.

          Detection of humsn CTNNBL1 by paraffin sections. Sample: Formalin-fixed paraffin-embedded human testis. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2
          Positive controlHeLa cells, testes tissue
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (40 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA synthtic polypeptide corresponding to amino acids 210-311 of human rCTNNBL1
          ImmunogenNA
          Clone5F1
          HostMouse
          IsotypeIgG3
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial, thaw aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-CTNNBL1 Mouse mAb (5F1) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-CTNNBL1 Mouse mAb (5F1) Certificates of Analysis

          TitleLot Number
          AP1135
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-October-2010 JLN
          SynonymsAnti-Beta catenin like protein 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (40 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human CTNNBL1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CTNNBL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (40 µg/ml). Detection: fluorescence.

          Detection of humsn CTNNBL1 by paraffin sections. Sample: Formalin-fixed paraffin-embedded human testis. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~65-70 kDa CTNNBL1 protein.
          BackgroundCTNNBL1 (Beta-catenin-like protein 1) is an apoptosis inducing protein with a structural homology similar to that of beta-catenin. CTNNBL1 is highly conservered across various species and it is primarily expressed in the nucleus of various tissues. In human, CTNNBL1 shows highest expression in skeletal muscle, placenta, heart, spleen, testis, and thyroid tissues. CTNNBL1 has been linked to a possible role in the Wnt/beta-catenin-signaling pathway and may have a function similar to that of beta catenin.
          HostMouse
          Immunogen speciesNA
          ImmunogenA synthtic polypeptide corresponding to amino acids 210-311 of human rCTNNBL1
          Clone5F1
          IsotypeIgG3
          Specieshuman
          Positive controlHeLa cells, testes tissue
          Formliquid
          FormulationIn PBS, pH 7.2
          PreservativeNone
          CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial, thaw aliquot and freeze (-20°C).
          Toxicity Regulatory Review