07-729 Anti-CTCF Antibody

07-729
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Ca, M, Pm WB, ChIP-seq, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-729
      ReplacesMABE547
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CTCF Antibody
      Alternate Names
      • 11 zinc finger transcriptional repressor
      • 11-zinc finger protein
      • CCCTC-binding factor
      • CCCTC-binding factor (zinc finger protein)
      • CTCFL paralog
      • transcriptional repressor CTCF
      Background InformationCTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
      References
      Product Information
      FormatSerum
      Control
      • HeLa nuclear extract.

        Included Positive Antigen Control:
        Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
      PresentationRabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
      ConcentrationThe concentration of this serum antibody has not been determined.
      HostRabbit
      SpecificityRecognizes CTCF at a.a. 659-675.
      Species Reactivity
      • Human
      • Rat
      • Canine
      • Mouse
      • Primate
      Species Reactivity NoteHuman. Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
      Gene Symbol
      • CTCF
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION:SwissProt: P49711 # Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.
      SIZE: 727 amino acids; 82785 Da
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.
      SIMILARITY: SwissProt: P49711 ## Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.
      Molecular WeightApprox. 120 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot.

      Western Blot Analysis:
      A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CTCF2475698
      Anti-CTCF (rabbit antiserum) - DAM1472197DAM1472197
      Anti-CTCF (rabbit antiserum) Polyclonal AntibodyQ2912988
      Anti-CTCF - 21422322142232
      Anti-CTCF - 23756062375606
      Anti-CTCF - 24524972452497
      Anti-CTCF - 13506371350637
      Anti-CTCF - 19621171962117
      Anti-CTCF - 20160342016034
      Anti-CTCF - 20545232054523

      References

      Reference overviewApplicationSpeciesPub Med ID
      Tissue-specific regulation and function of Grb10 during growth and neuronal commitment.
      Plasschaert, RN; Bartolomei, MS
      Proceedings of the National Academy of Sciences of the United States of America  112  6841-7  2015

      Show Abstract
      25368187 25368187
      Epigenetic silencing of the XAF1 gene is mediated by the loss of CTCF binding.
      Victoria-Acosta, G; Vazquez-Santillan, K; Jimenez-Hernandez, L; Muñoz-Galindo, L; Maldonado, V; Martinez-Ruiz, GU; Melendez-Zajgla, J
      Scientific reports  5  14838  2015

      Show Abstract
      26443201 26443201
      Genome-wide targeting of the epigenetic regulatory protein CTCF to gene promoters by the transcription factor TFII-I.
      Peña-Hernández, R; Marques, M; Hilmi, K; Zhao, T; Saad, A; Alaoui-Jamali, MA; del Rincon, SV; Ashworth, T; Roy, AL; Emerson, BM; Witcher, M
      Proceedings of the National Academy of Sciences of the United States of America  112  E677-86  2015

      Show Abstract
      25646466 25646466
      The contribution of cohesin-SA1 to gene expression and chromatin architecture in two murine tissues.
      Cuadrado, A; Remeseiro, S; Graña, O; Pisano, DG; Losada, A
      Nucleic acids research  43  3056-67  2015

      Show Abstract
      25735743 25735743
      T-cell receptor α enhancer is inactivated in αβ T lymphocytes.
      del Blanco, B; Angulo, Ú; Krangel, MS; Hernández-Munain, C
      Proceedings of the National Academy of Sciences of the United States of America  112  E1744-53  2015

      Show Abstract
      Immunoprecipitation25831496 25831496
      Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators.
      Mehta, K; Gunasekharan, V; Satsuka, A; Laimins, LA
      PLoS pathogens  11  e1004763  2015

      Show Abstract
      25875106 25875106
      Epigenomic evolution in diffuse large B-cell lymphomas.
      Pan, H; Jiang, Y; Boi, M; Tabbò, F; Redmond, D; Nie, K; Ladetto, M; Chiappella, A; Cerchietti, L; Shaknovich, R; Melnick, AM; Inghirami, GG; Tam, W; Elemento, O
      Nature communications  6  6921  2015

      Show Abstract
      25891015 25891015
      Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation.
      Chen, K; Hu, J; Moore, DL; Liu, R; Kessans, SA; Breslin, K; Lucet, IS; Keniry, A; Leong, HS; Parish, CL; Hilton, DJ; Lemmers, RJ; van der Maarel, SM; Czabotar, PE; Dobson, RC; Ritchie, ME; Kay, GF; Murphy, JM; Blewitt, ME
      Proceedings of the National Academy of Sciences of the United States of America  112  E3535-44  2015

      Show Abstract
      26091879 26091879
      RBPJ, the major transcriptional effector of Notch signaling, remains associated with chromatin throughout mitosis, suggesting a role in mitotic bookmarking.
      Lake, RJ; Tsai, PF; Choi, I; Won, KJ; Fan, HY
      PLoS genetics  10  e1004204  2014

      Show Abstract
      Western Blotting24603501 24603501
      Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation.
      Günal-Sadık, G; Paszkowski-Rogacz, M; Singaravelu, K; Beyer, A; Buchholz, F; Jessberger, R
      PloS one  9  e111748  2014

      Show Abstract
      25375358 25375358

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

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      Included Positive Control

      Catalog Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies