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MAB326R | Anti-CNPase Antibody, clone 11-5B

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, M, Po, R, Rb, Sh ICC, IHC, IH(P), WB M Purified Monoclonal Antibody
      Catalogue NumberMAB326R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CNPase Antibody, clone 11-5B
      Background InformationThe enzyme 2', 3'-cyclic nucleotide 3'-phosphodi-esterase (CNP) is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system (Sprinkle, 1989). By virtue of this cell-specific expression, CNP is recognized as a characteristic marker for these two myelin-producing glial cell types (Sprinkle, 1989; Kim et al., 1984; Sheedlo & Sprinkle, 1984; McMorris et al., 1984). Beyond its enzymatic activity of cleaving the 2', 3'-cyclic terminus of nucleotides (Sprinkle, 1989), the physiological role of CNP is still under investigation. CNP activity has been correlated with myelin and myelin formation, and a dramatic decrease in CNP activity is associated with demyelinating diseases such as multiple sclerosis (Sprinkle, 1989). This enzyme is composed of two proteins, CNP1 (46 kD) and CNP2 (48 kD) (Sprinkle, 1989; Sprinkle et al., 1987). Although the ratio of CNP1/CNP2 may vary from species to species, their shared primary sequence is conserved phyIogenetically. CNP has recently been localized to human chromosome 17 by amplification of somatic cell hybrid DNA using the polymerase chain reaction (PCR) and by Southern blotting of Hind Ill genomic DNA digests (Sprinkle et al., 1991). Since anti-CNP reacts with a highly conserved region of the enzyme, it can be considered as pan-anti-CNP (Sprinkle et al., 1987). Anti-CNP can be used as a marker to identify Schwann cells and oligodendrocytes in cell cuIture and in tissue sections, as well as to localize CNP in cell membrane fractions. As CNP is expressed relatively early in postnatal development, anti-CNP is especially useful for the early identification of oligodendrocytes.
      Product Information
      • Oligodendrocyte culture, Brain lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, pH 7.6, 0.25M NaCl with 0.1% sodium azide.
      ApplicationThis Anti-CNPase Antibody, clone 11-5B is validated for use in IC, IH, IH(P), WB for the detection of CNPase.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used in IH (10 μg/mL antibody, prepared fresh daily).

      A previous lot of this antibody was used in IC (10 μg/mL antibody, prepared fresh daily).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified human 2’, 3’-cyclic nucleotide 3’-phosphodiesterase
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAnti-CNP reacts with both CNP1 and CNP2 in many species.
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CNP
      • CNPase
      • CNP1
      • EC
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummarySIZE: 421 amino acids; 47579 Da
      SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
      Molecular Weight48 & 46 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse brain lysates.

      Western Blotting Analysis:
      1:500 dilution of this antibody detected CNPASE 1/2 on 10 µg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CNPase, clone 11-5B - 2368742 2368742
      Anti-CNPase, clone 11-5B - 2433515 2433515
      Anti-CNPase, clone 11-5B - 1994902 1994902
      Anti-CNPase, clone 11-5B - 2029624 2029624
      Anti-CNPase, clone 11-5B - 2090117 2090117
      Anti-CNPase, clone 11-5B - 2207167 2207167
      Anti-CNPase, clone 11-5B - 2293844 2293844
      Anti-CNPase, clone 11-5B - LV1547626 LV1547626
      Anti-CNPase, clone 11-5B - LV1638248 LV1638248
      Anti-CNPase, clone 11-5B - LV1679493 LV1679493
      Anti-CNPase, clone 11-5B - NG1849448 NG1849448
      Anti-CNPase, clone 11-5B -2489025 2489025
      Anti-CNPase, clone 11-5B -2567060 2567060
      Anti-CNPase, clone 11-5B -2607260 2607260
      Anti-CNPase, clone 11-5B -2726802 2726802
      Anti-CNPase, clone 11-5B -2768411 2768411

      References | 21 Available | See All References

      Reference overviewApplicationPub Med ID
      Transient Blockade of ERK Phosphorylation in the Critical Period Causes Autistic Phenotypes as an Adult in Mice.
      Yufune, S; Satoh, Y; Takamatsu, I; Ohta, H; Kobayashi, Y; Takaenoki, Y; Pagès, G; Pouysségur, J; Endo, S; Kazama, T
      Scientific reports 5 10252 2015

      Show Abstract
      25993696 25993696
      Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth.
      Verginelli, F; Perin, A; Dali, R; Fung, KH; Lo, R; Longatti, P; Guiot, MC; Del Maestro, RF; Rossi, S; di Porzio, U; Stechishin, O; Weiss, S; Stifani, S
      Nature communications 4 2956 2013

      Show Abstract
      24356439 24356439
      Application of immunohistochemistry in stereology for quantitative assessment of neural cell populations illustrated in the Göttingen minipig.
      Hou, J; Riise, J; Pakkenberg, B
      PloS one 7 e43556 2012

      Show Abstract
      22905271 22905271
      Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.
      Deng, HX; Chen, W; Hong, ST; Boycott, KM; Gorrie, GH; Siddique, N; Yang, Y; Fecto, F; Shi, Y; Zhai, H; Jiang, H; Hirano, M; Rampersaud, E; Jansen, GH; Donkervoort, S; Bigio, EH; Brooks, BR; Ajroud, K; Sufit, RL; Haines, JL; Mugnaini, E; Pericak-Vance, MA; Siddique, T
      Nature 477 211-5 2011

      Show Abstract
      21857683 21857683
      Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model.
      Vergo, S; Craner, MJ; Etzensperger, R; Attfield, K; Friese, MA; Newcombe, J; Esiri, M; Fugger, L
      Brain : a journal of neurology 134 571-84 2011

      Show Abstract
      21233144 21233144
      A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons.
      Zhang, J; Giesert, F; Kloos, K; Vogt Weisenhorn, DM; Aigner, L; Wurst, W; Couillard-Despres, S
      BMC neuroscience 11 158 2010

      Show Abstract
      Immunohistochemistry21194452 21194452
      Neuronal differentiation of NG108-15 cells has impact on nitric oxide- and membrane (natriuretic peptide receptor-A) cyclic GMP-generating proteins.
      Dieter Müller,Karen J Greenland,Robert C Speth,Ralf Middendorff
      Molecular and cellular endocrinology 320 2010

      Show Abstract
      20097258 20097258
      Bone marrow stromal cell therapy reduces proNGF and p75 expression in mice with experimental autoimmune encephalomyelitis.
      Zhang, J; Brodie, C; Li, Y; Zheng, X; Roberts, C; Lu, M; Gao, Q; Borneman, J; Savant-Bhonsale, S; Elias, SB; Chopp, M
      Journal of the neurological sciences 279 30-8 2009

      Show Abstract
      Immunohistochemistry19193386 19193386
      Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.
      Lyck, L; Dalmau, I; Chemnitz, J; Finsen, B; Schrøder, HD
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56 201-21 2008

      Show Abstract Full Text Article
      17998570 17998570
      Corticosteroids reverse cytokine-induced block of survival and differentiation of oligodendrocyte progenitor cells from rats.
      Mann, SA; Versmold, B; Marx, R; Stahlhofen, S; Dietzel, ID; Heumann, R; Berger, R
      Journal of neuroinflammation 5 39 2008

      Show Abstract Full Text Article
      18808689 18808689

      Data Sheet

      Anti-CNPase, clone 11-5B - Data Sheet

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