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MABS444 | Anti-CLCA1 Antibody, clone 4D2.2

MABS444
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS444
      DescriptionAnti-CLCA1 Antibody, clone 4D2.2
      Alternate Names
      • Calcium-activated chloride channel regulator 1
      • Calcium-activated chloride channel family member 1
      • Calcium-activated chloride channel protein 1
      • CaCC-1
      • hCaCC-1
      • hCLCA1
      Background InformationCalcium-activated chloride channel regulator 1 (UniProt A8K7I4; also known as CaCC-1, Calcium-activated chloride channel family member 1, Calcium-activated chloride channel protein 1, hCaCC-1, hCLCA1) is encoded by the CLCA1 (also known as CACC, CACC1, CLCC1, GOB5) gene (Gene ID 1179) in human. CLCA1 belongs to the calcium-activated chloride channel regulator (previously known as chloride channel calcium activated or CLCA) family of self-cleaving, secreted metalloproteases that activate calcium-dependent chloride currents (ICaCC) in mammalian cells. Originally thought to be calcium-activated chloride channels (CaCCs), CLCA proteins were later identified as secreted CaCCs modulators. TMEM16A (a.k.a. Anoctamin1 and DOG1) is a CaCC expressed in airway epithelia and smooth muscle. The secreted CLCA1 is reported to physically interact with TMEM16A and modulate TMEM16A-dependent calcium-activated chloride currents. Adding CLCA1 to cells causes more TMEM16A channels to appear in the cell membrane, which increases the flow of chloride ions.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CLCA1 Antibody, clone 4D2.2 is an antibody against CLCA1 for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CLCA1 in human small intestine and rat testis tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to an internal fragment from the C-terminal half of human CLCA1.
      EpitopeInternal (C-terminal half).
      Clone4D2.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityDetects the 90 kDa processed/cleaved 4-transmembrane glycoprotein, but not the cleaved 37-41 kDa C-terminal fragment.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLCA1
      • CACC
      • CACC1
      • CLCC1
      • GOB5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~83 kDa observed. 125 kDa glycosylated precursor form, 90 kDa and 37-41 kDa processed/cleaved fragements reported (Gruber, A.D., et al. (1998). Genomics. 54(2):200-214).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CLCA1 in 10 µg of human small intestine tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications