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07-057 | Anti-CHK2/Cds1 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Polyclonal Antibody
      Catalogue Number07-057
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CHK2/Cds1 Antibody
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationDetect CHK2/Cds1 with Anti-CHK2/Cds1 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenFull-length human recombinant CHK2/Cds1
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • CHEK2
      • bA444G7
      • RAD53
      • OTTHUMP00000028871
      • HuCds1
      • CHK2
      • PP1425
      • Cds1
      • LFS2
      • CDS1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
      COFACTOR: Magnesium.
      SIZE: 543 amino acids; 60915 Da
      SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
      TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
      DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
      SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.
      Molecular Weight65kDa; appears as a doublet on longer exposures
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on 293 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CHK2/Cds1 (rabbit polyclonal IgG) - 2436352 2436352
      Anti-CHK2/Cds1 (rabbit polyclonal IgG) - 2183664 2183664
      Anti-CHK2/Cds1 - 20056 20056
      Anti-CHK2/Cds1 - 2040528 2040528
      Anti-CHK2/Cds1 - 2073117 2073117
      Anti-CHK2/Cds1 - 28844 28844


      Reference overviewApplicationPub Med ID
      MicroRNA-138 modulates DNA damage response by repressing histone H2AX expression.
      Wang, Y; Huang, JW; Li, M; Cavenee, WK; Mitchell, PS; Zhou, X; Tewari, M; Furnari, FB; Taniguchi, T
      Molecular cancer research : MCR 9 1100-11 2011

      Show Abstract
      21693595 21693595
      Stage-Specific Modulation of Cortical Neuronal Development by Mmu-miR-134.
      Gaughwin P, Ciesla M, Yang H, Lim B, Brundin P
      Cereb Cortex 2011

      Show Abstract
      21228099 21228099
      The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase.
      Oliva-Trastoy, M; Berthonaud, V; Chevalier, A; Ducrot, C; Marsolier-Kergoat, MC; Mann, C; Leteurtre, F
      Oncogene 26 1449-58 2007

      Show Abstract
      16936775 16936775
      Molecular basis for G2 arrest induced by 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine and consequences of checkpoint abrogation.
      Liu, X; Guo, Y; Li, Y; Jiang, Y; Chubb, S; Azuma, A; Huang, P; Matsuda, A; Hittelman, W; Plunkett, W
      Cancer research 65 6874-81 2005

      Show Abstract
      16061671 16061671
      Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay
      Shibata, Y. and Nakamura, T.
      J Biol Chem, 277:746-54 (2002) 2002

      Immunoblotting (Western)11687589 11687589
      A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage.
      Brown, A L, et al.
      Proc. Natl. Acad. Sci. U.S.A., 96: 3745-50 (1999) 1999

      Show Abstract
      10097108 10097108

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