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ABE1307 | Anti-CHD1L Antibody

ABE1307
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE1307
      DescriptionAnti-CHD1L Antibody
      Alternate Names
      • Chromodomain-helicase-DNA-binding protein 1-like
      • Amplified in liver cancer protein 1
      • CHD1L
      Background InformationChromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) is a ~100.9 kDa DNA helicase that is amplified in many solid tumors. The CHD1L gene, located at Chr 1q21 is frequently amplified in hepatocellular carcinoma. CHD1L plays a role in chromatin remodeling following DNA damage. It is targeted to DNA damage sites through interaction with poly(ADP)-ribose) and regulated chromatin during DNA repair. CHD1L contains a SNF2-N domain and a helicase superfamily domain, but unlike CHD1, it does not contain a chromodomain, but instead contains a c-terminal macrodomain, which is an (ADP)-ribose/ polymer of the ADP-ribose (PAR)-binding element. In addition to PAR binding, the macro domain of CHD1L is able to bind Nur77, a critical member of a p53-independent apoptotic pathway. This binding subsequently inhibits the nucleus-to-mitochondria translocation of Nur77 and disrupts apoptotic cell death. Overexpression of CHD1L protein in tumors is considered to be an independent biomarker of poor prognosis and short tumor-free survival time.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CHD1L Antibody is an antibody against CHD1L for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected CHD1L in 10 µg of Jurkat extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the Helicase C-terminal domain of human CHD1L.
      EpitopeHelicase C-terminal domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis isoform has been observed to react with isoform 1 and 3.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHD1L
      • ALC1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~78/110 kDa observed. Uncharacterized band(s) may appear in some lysates. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CHD1L in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications