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MABT325 | Anti-CEACAM5 Antibody, clone 5C8C4

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, FC, ICC, IH(P), IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMABT325
      DescriptionAnti-CEACAM5 Antibody, clone 5C8C4
      Alternate Names
      • CEACAM5
      • Carcinoembryonic antigen-related cell adhesion molecule 5
      • Carcinoembryonic antigen
      • CEA
      • Meconium antigen 100
      • CD66e
      Background InformationThe carcinoembryonic antigen- (CEA-) related cell adhesion molecules (CEACAMs) constitute a 12-member subgroup of the CEA family of immunoglobulin-related proteins first described in 1965 (PMID 4953873). CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules from neighbouring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEACAM molecules within the same cell can also undergo transmembrane domain-mediated cis-oligomerization, an event important for sustaining downstream cellular signaling. Athough CEACAM1 is shown to utilize its cytoplasmic domain for transducing cellular signaling, not all CEACAM members are expressed with a significant cytoplasmic domain, and others (CEACAM5/6/7/8) are GPI-anchored without even a transmembrane domain. (PMID 4735478). Human CEACAM5 (also known as CD66e, CEA, Ceacam5, Meconium antigen 100; UniProt P06731) is encoded by the CEACAM5 (or CEA) gene (NCBI Gene ID 1048). It is a GPI-anchored CEACAM with the most extracellular Ig domains, 7 in all.
      Product Information
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-CEACAM5 Antibody, clone 5C8C4 is an antibody against CEACAM5 for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CEACAM5 in human lung cancer and breast cancer tissue sections.

      Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated CEACAM5 in human granulocytes lysate (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

      Western Blotting Analysis: 10 µg/mL from a representative lot detected CEACAM5 in human granulocyte lysate (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

      ELISA Analysis: A representative lot detected CEACAM5 in bronchoalveolar lavage fluid and NHBE cells (Klaile, E., et al. (2013). Respir. Res. 14:85).

      Flow Cytometry Analysis: A representative lot detected the exogenously expressed human CEACAM5 on the surface of transfected HeLa cells (Gutbier, B., et al. (2015). Am. J. Physiol. Lung Cell Mol. Physiol. 309(3):L250-261).

      Flow Cytometry Analysis: A representative lot detected CEACAM5 in NHBE cell surfaces (Klaile, E., et al. (2013). Respir. Res. 14:85).

      Immunocytochemistry Analysis: A representative lot detected CEACAM5 in non-permeabilized NHBE cells (Klaile, E., et al. (2013). Respir. Res. 14:85).

      Immunohistochemistry Analysis: A representative lot detected CEACAM5 in paraffin sections of human lung tissue (Klaile, E., et al. (2013). Respir. Res. 14:85).

      Western Blotting Analysis: A representative lot detected CEACAM5 in human epithelial cell (HT29, T102/3), human
      endothelial cells (AS-M.5), mouse endothelial cells (bEND3), CHO and CHO-CEACAM1 cells (Muturi, H.T., et al. (2013). PLoS One. 8(9):e74654).
      Biological Information
      ImmunogenRecombinant human CEACAM5 extracellular domain.
      Epitopeextracellular domain
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEACAM5
      • CEA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~180 kDa observed. Target band size appears larger than the calculated molecular weights of 71.33/76.80 kDa (mature/pro-form) due to glycosylation.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human colon cancer tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected CEACAM5 in human colon cancer tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies